The Immunotherapeutic Effect of SIRPα-Silenced DCs against Cervical Cancer

Signal regulatory protein α (SIRPα), a transmembrane protein that is predominantly expressed in dendritic cells (DCs) or macrophages, interacts with CD47 that is overexpressed in almost all types of tumor cells. The interaction between SIRPα and CD47 leads to a negative signal that prevents the phen...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Xiaojie, Zhou, Wenying, Liang, Yanlan, Xu, Changzhi, Xie, Zhizhi, Liang, Jiayin, Hu, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7199593/
https://www.ncbi.nlm.nih.gov/pubmed/32411788
http://dx.doi.org/10.1155/2020/1705187
_version_ 1783529176988385280
author Li, Xiaojie
Zhou, Wenying
Liang, Yanlan
Xu, Changzhi
Xie, Zhizhi
Liang, Jiayin
Hu, Bo
author_facet Li, Xiaojie
Zhou, Wenying
Liang, Yanlan
Xu, Changzhi
Xie, Zhizhi
Liang, Jiayin
Hu, Bo
author_sort Li, Xiaojie
collection PubMed
description Signal regulatory protein α (SIRPα), a transmembrane protein that is predominantly expressed in dendritic cells (DCs) or macrophages, interacts with CD47 that is overexpressed in almost all types of tumor cells. The interaction between SIRPα and CD47 leads to a negative signal that prevents the phenotypic and functional maturation of DC and inhibits phagocytosis. The SIRPα knockdown in DCs that were pulsed with a modified HPV16E7 (HPV16mE7) protein with enhanced antigenicity and reduced transformation activity results in increased cytokine (TNF-α/IL-12/IL-6) secretion, IFN-γ secretion by T lymphocytes, and in vitro/in vivo tumoricidal activity against cervical cancer cells. Taken together, these results suggest that SIRPα-silenced DC vaccination presented potential therapeutic implications against cervical cancer.
format Online
Article
Text
id pubmed-7199593
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-71995932020-05-14 The Immunotherapeutic Effect of SIRPα-Silenced DCs against Cervical Cancer Li, Xiaojie Zhou, Wenying Liang, Yanlan Xu, Changzhi Xie, Zhizhi Liang, Jiayin Hu, Bo J Immunol Res Research Article Signal regulatory protein α (SIRPα), a transmembrane protein that is predominantly expressed in dendritic cells (DCs) or macrophages, interacts with CD47 that is overexpressed in almost all types of tumor cells. The interaction between SIRPα and CD47 leads to a negative signal that prevents the phenotypic and functional maturation of DC and inhibits phagocytosis. The SIRPα knockdown in DCs that were pulsed with a modified HPV16E7 (HPV16mE7) protein with enhanced antigenicity and reduced transformation activity results in increased cytokine (TNF-α/IL-12/IL-6) secretion, IFN-γ secretion by T lymphocytes, and in vitro/in vivo tumoricidal activity against cervical cancer cells. Taken together, these results suggest that SIRPα-silenced DC vaccination presented potential therapeutic implications against cervical cancer. Hindawi 2020-01-07 /pmc/articles/PMC7199593/ /pubmed/32411788 http://dx.doi.org/10.1155/2020/1705187 Text en Copyright © 2020 Xiaojie Li et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Xiaojie
Zhou, Wenying
Liang, Yanlan
Xu, Changzhi
Xie, Zhizhi
Liang, Jiayin
Hu, Bo
The Immunotherapeutic Effect of SIRPα-Silenced DCs against Cervical Cancer
title The Immunotherapeutic Effect of SIRPα-Silenced DCs against Cervical Cancer
title_full The Immunotherapeutic Effect of SIRPα-Silenced DCs against Cervical Cancer
title_fullStr The Immunotherapeutic Effect of SIRPα-Silenced DCs against Cervical Cancer
title_full_unstemmed The Immunotherapeutic Effect of SIRPα-Silenced DCs against Cervical Cancer
title_short The Immunotherapeutic Effect of SIRPα-Silenced DCs against Cervical Cancer
title_sort immunotherapeutic effect of sirpα-silenced dcs against cervical cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7199593/
https://www.ncbi.nlm.nih.gov/pubmed/32411788
http://dx.doi.org/10.1155/2020/1705187
work_keys_str_mv AT lixiaojie theimmunotherapeuticeffectofsirpasilenceddcsagainstcervicalcancer
AT zhouwenying theimmunotherapeuticeffectofsirpasilenceddcsagainstcervicalcancer
AT liangyanlan theimmunotherapeuticeffectofsirpasilenceddcsagainstcervicalcancer
AT xuchangzhi theimmunotherapeuticeffectofsirpasilenceddcsagainstcervicalcancer
AT xiezhizhi theimmunotherapeuticeffectofsirpasilenceddcsagainstcervicalcancer
AT liangjiayin theimmunotherapeuticeffectofsirpasilenceddcsagainstcervicalcancer
AT hubo theimmunotherapeuticeffectofsirpasilenceddcsagainstcervicalcancer
AT lixiaojie immunotherapeuticeffectofsirpasilenceddcsagainstcervicalcancer
AT zhouwenying immunotherapeuticeffectofsirpasilenceddcsagainstcervicalcancer
AT liangyanlan immunotherapeuticeffectofsirpasilenceddcsagainstcervicalcancer
AT xuchangzhi immunotherapeuticeffectofsirpasilenceddcsagainstcervicalcancer
AT xiezhizhi immunotherapeuticeffectofsirpasilenceddcsagainstcervicalcancer
AT liangjiayin immunotherapeuticeffectofsirpasilenceddcsagainstcervicalcancer
AT hubo immunotherapeuticeffectofsirpasilenceddcsagainstcervicalcancer

Ejemplares similares