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Generation of (34)S-substituted protein-bound [4Fe-4S] clusters using (34)S-L-cysteine
The ability to specifically label the sulphide ions of protein-bound iron–sulphur (FeS) clusters with (34)S isotope greatly facilitates structure–function studies. In particular, it provides insight when using either spectroscopic techniques that probe cluster-associated vibrations, or non-denaturin...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200944/ https://www.ncbi.nlm.nih.gov/pubmed/32395620 http://dx.doi.org/10.1093/biomethods/bpy015 |