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Fast and accurate defocus modulation for improved tunability of cryo-EM experiments

Current data collection strategies in electron cryo-microscopy (cryo-EM) record multiframe movies with static optical settings. This limits the number of adjustable parameters that can be used to optimize the experiment. Here, a method for fast and accurate defocus (FADE) modulation during movie acq...

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Detalles Bibliográficos
Autores principales: Danev, Radostin, Iijima, Hirofumi, Matsuzaki, Mizuki, Motoki, Sohei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201282/
https://www.ncbi.nlm.nih.gov/pubmed/32431839
http://dx.doi.org/10.1107/S205225252000408X