Improved designs for pET expression plasmids increase protein production yield in Escherichia coli

The pET series of expression plasmids are widely used for recombinant protein production in Escherichia coli. The genetic modules controlling transcription and translation in these plasmids were first described in the 1980s and have not changed since. Herein we report design flaws in these genetic m...

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Detalles Bibliográficos
Autores principales: Shilling, Patrick J., Mirzadeh, Kiavash, Cumming, Alister J., Widesheim, Magnus, Köck, Zoe, Daley, Daniel O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205610/
https://www.ncbi.nlm.nih.gov/pubmed/32382055
http://dx.doi.org/10.1038/s42003-020-0939-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205610/
https://www.ncbi.nlm.nih.gov/pubmed/32382055
http://dx.doi.org/10.1038/s42003-020-0939-8

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