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Improved designs for pET expression plasmids increase protein production yield in Escherichia coli
The pET series of expression plasmids are widely used for recombinant protein production in Escherichia coli. The genetic modules controlling transcription and translation in these plasmids were first described in the 1980s and have not changed since. Herein we report design flaws in these genetic m...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205610/ https://www.ncbi.nlm.nih.gov/pubmed/32382055 http://dx.doi.org/10.1038/s42003-020-0939-8 |