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A simple technique for suppressor detection inEscherichia coli
To study the viability of agyrA S83 stop mutation found in anEscherichia coli J53 ciprofloxacin-resistant strain (J53 CipR27), a pBR322 derivative was constructed with a TAG mutation in thebla gene knocking out ampicillin resistance. Ampicillin resistance was restored, suggesting that the strain con...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2016
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7207127/ https://www.ncbi.nlm.nih.gov/pubmed/27682418 http://dx.doi.org/10.1093/femsle/fnw228 |