A simple technique for suppressor detection inEscherichia coli

To study the viability of agyrA S83 stop mutation found in anEscherichia coli J53 ciprofloxacin-resistant strain (J53 CipR27), a pBR322 derivative was constructed with a TAG mutation in thebla gene knocking out ampicillin resistance. Ampicillin resistance was restored, suggesting that the strain con...

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Autores principales: Vinué, Laura, Hooper, David C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7207127/
https://www.ncbi.nlm.nih.gov/pubmed/27682418
http://dx.doi.org/10.1093/femsle/fnw228
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author Vinué, Laura
Hooper, David C.
author_facet Vinué, Laura
Hooper, David C.
author_sort Vinué, Laura
collection PubMed
description To study the viability of agyrA S83 stop mutation found in anEscherichia coli J53 ciprofloxacin-resistant strain (J53 CipR27), a pBR322 derivative was constructed with a TAG mutation in thebla gene knocking out ampicillin resistance. Ampicillin resistance was restored, suggesting that the strain contains tRNA suppressor activity able to suppress the UAG codongyrA and allow viability. The method was applied to 22 unique clinicalE. coli isolates, and all were found to have low-level suppressor activity.
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spelling pubmed-72071272020-05-13 A simple technique for suppressor detection inEscherichia coli Vinué, Laura Hooper, David C. FEMS Microbiol Lett Letter to the Editor To study the viability of agyrA S83 stop mutation found in anEscherichia coli J53 ciprofloxacin-resistant strain (J53 CipR27), a pBR322 derivative was constructed with a TAG mutation in thebla gene knocking out ampicillin resistance. Ampicillin resistance was restored, suggesting that the strain contains tRNA suppressor activity able to suppress the UAG codongyrA and allow viability. The method was applied to 22 unique clinicalE. coli isolates, and all were found to have low-level suppressor activity. Oxford University Press 2016-09-27 2016-10-01 /pmc/articles/PMC7207127/ /pubmed/27682418 http://dx.doi.org/10.1093/femsle/fnw228 Text en © FEMS 2016. All rights reserved. For permissions, please e-mail:journals.permissions@oup.com http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Letter to the Editor
Vinué, Laura
Hooper, David C.
A simple technique for suppressor detection inEscherichia coli
title A simple technique for suppressor detection inEscherichia coli
title_full A simple technique for suppressor detection inEscherichia coli
title_fullStr A simple technique for suppressor detection inEscherichia coli
title_full_unstemmed A simple technique for suppressor detection inEscherichia coli
title_short A simple technique for suppressor detection inEscherichia coli
title_sort simple technique for suppressor detection inescherichia coli
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7207127/
https://www.ncbi.nlm.nih.gov/pubmed/27682418
http://dx.doi.org/10.1093/femsle/fnw228
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