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Development and Standardization of a High-Throughput Bordetella pertussis Growth-Inhibition Assay
Bordetella pertussis, the main causative agent of whooping cough, is a reemerging pathogen, and recent vaccine-resistant strain outbreaks and emergence of macrolides-resistant strains in China raised new concerns for control of the disease. New vaccines and potentially new antibiotics are thus neede...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212404/ https://www.ncbi.nlm.nih.gov/pubmed/32425912 http://dx.doi.org/10.3389/fmicb.2020.00777 |
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author | Thiriard, Anaïs Raze, Dominique Locht, Camille |
author_facet | Thiriard, Anaïs Raze, Dominique Locht, Camille |
author_sort | Thiriard, Anaïs |
collection | PubMed |
description | Bordetella pertussis, the main causative agent of whooping cough, is a reemerging pathogen, and recent vaccine-resistant strain outbreaks and emergence of macrolides-resistant strains in China raised new concerns for control of the disease. New vaccines and potentially new antibiotics are thus needed. B. pertussis is tedious to culture and requires several days of growth to count isolated colonies on agar-based media, making large-scale screening of new anti-B. pertussis compounds or functional evaluation of large sample sizes of immune sera difficult. Here, we developed a scalable, rapid, high-throughput luminescence-based Bordetella growth inhibition assay (BGIA) to quantify surviving bacteria after treatment with anti-B. pertussis compounds. A strong correlation between luminescence and colony-forming units (r(2) = 0.9345, p < 0.0001) was found and the BGIA showed high sensitivity and reproducibility. We demonstrate here that the BGIA can be used to quantify resistance of B. pertussis to antibiotics, sensitivity to complement and to human serum in an easy-to-operate and fast manner. We have optimized the assay and tested the effects of different B. pertussis strains and growth conditions on serum and complement sensitivity. We also uncovered complement-independent antibody-mediated inhibition of B. pertussis growth. The BGIA can thus effectively be implemented for large-scale serum studies to further investigate anti-B. pertussis immune responses at a functional level, as well as for screening of B. pertussis strains for their resistance to antibiotics or complement, and for high-throughput screening of novel anti-B. pertussis compounds. |
format | Online Article Text |
id | pubmed-7212404 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72124042020-05-18 Development and Standardization of a High-Throughput Bordetella pertussis Growth-Inhibition Assay Thiriard, Anaïs Raze, Dominique Locht, Camille Front Microbiol Microbiology Bordetella pertussis, the main causative agent of whooping cough, is a reemerging pathogen, and recent vaccine-resistant strain outbreaks and emergence of macrolides-resistant strains in China raised new concerns for control of the disease. New vaccines and potentially new antibiotics are thus needed. B. pertussis is tedious to culture and requires several days of growth to count isolated colonies on agar-based media, making large-scale screening of new anti-B. pertussis compounds or functional evaluation of large sample sizes of immune sera difficult. Here, we developed a scalable, rapid, high-throughput luminescence-based Bordetella growth inhibition assay (BGIA) to quantify surviving bacteria after treatment with anti-B. pertussis compounds. A strong correlation between luminescence and colony-forming units (r(2) = 0.9345, p < 0.0001) was found and the BGIA showed high sensitivity and reproducibility. We demonstrate here that the BGIA can be used to quantify resistance of B. pertussis to antibiotics, sensitivity to complement and to human serum in an easy-to-operate and fast manner. We have optimized the assay and tested the effects of different B. pertussis strains and growth conditions on serum and complement sensitivity. We also uncovered complement-independent antibody-mediated inhibition of B. pertussis growth. The BGIA can thus effectively be implemented for large-scale serum studies to further investigate anti-B. pertussis immune responses at a functional level, as well as for screening of B. pertussis strains for their resistance to antibiotics or complement, and for high-throughput screening of novel anti-B. pertussis compounds. Frontiers Media S.A. 2020-04-23 /pmc/articles/PMC7212404/ /pubmed/32425912 http://dx.doi.org/10.3389/fmicb.2020.00777 Text en Copyright © 2020 Thiriard, Raze and Locht. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Thiriard, Anaïs Raze, Dominique Locht, Camille Development and Standardization of a High-Throughput Bordetella pertussis Growth-Inhibition Assay |
title | Development and Standardization of a High-Throughput Bordetella pertussis Growth-Inhibition Assay |
title_full | Development and Standardization of a High-Throughput Bordetella pertussis Growth-Inhibition Assay |
title_fullStr | Development and Standardization of a High-Throughput Bordetella pertussis Growth-Inhibition Assay |
title_full_unstemmed | Development and Standardization of a High-Throughput Bordetella pertussis Growth-Inhibition Assay |
title_short | Development and Standardization of a High-Throughput Bordetella pertussis Growth-Inhibition Assay |
title_sort | development and standardization of a high-throughput bordetella pertussis growth-inhibition assay |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212404/ https://www.ncbi.nlm.nih.gov/pubmed/32425912 http://dx.doi.org/10.3389/fmicb.2020.00777 |
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