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Non-AUG Translation Initiation Generates Peroxisomal Isoforms of 6-Phosphogluconate Dehydrogenase in Fungi

Proteins destined for transport to specific organelles usually contain targeting information, which are embedded in their sequence. Many enzymes are required in more than one cellular compartment and different molecular mechanisms are used to achieve dual localization. Here we report a cryptic type...

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Autores principales: Kremp, Marco, Bittner, Elena, Martorana, Domenica, Klingenberger, Alexander, Stehlik, Thorsten, Bölker, Michael, Freitag, Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214817/
https://www.ncbi.nlm.nih.gov/pubmed/32432107
http://dx.doi.org/10.3389/fcell.2020.00251
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author Kremp, Marco
Bittner, Elena
Martorana, Domenica
Klingenberger, Alexander
Stehlik, Thorsten
Bölker, Michael
Freitag, Johannes
author_facet Kremp, Marco
Bittner, Elena
Martorana, Domenica
Klingenberger, Alexander
Stehlik, Thorsten
Bölker, Michael
Freitag, Johannes
author_sort Kremp, Marco
collection PubMed
description Proteins destined for transport to specific organelles usually contain targeting information, which are embedded in their sequence. Many enzymes are required in more than one cellular compartment and different molecular mechanisms are used to achieve dual localization. Here we report a cryptic type 2 peroxisomal targeting signal encoded in the 5′ untranslated region of fungal genes coding for 6-phosphogluconate dehydrogenase (PGD), a key enzyme of the oxidative pentose phosphate pathway. The conservation of the cryptic PTS2 motif suggests a biological function. We observed that translation from a non-AUG start codon generates an N-terminally extended peroxisomal isoform of Ustilago maydis PGD. Non-canonical initiation occurred at the sequence AGG AUU, consisting of two near-cognate start codons in tandem. Taken together, our data reveal non-AUG translation initiation as an additional mechanism to achieve the dual localization of a protein required both in the cytosol and the peroxisomes.
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spelling pubmed-72148172020-05-19 Non-AUG Translation Initiation Generates Peroxisomal Isoforms of 6-Phosphogluconate Dehydrogenase in Fungi Kremp, Marco Bittner, Elena Martorana, Domenica Klingenberger, Alexander Stehlik, Thorsten Bölker, Michael Freitag, Johannes Front Cell Dev Biol Cell and Developmental Biology Proteins destined for transport to specific organelles usually contain targeting information, which are embedded in their sequence. Many enzymes are required in more than one cellular compartment and different molecular mechanisms are used to achieve dual localization. Here we report a cryptic type 2 peroxisomal targeting signal encoded in the 5′ untranslated region of fungal genes coding for 6-phosphogluconate dehydrogenase (PGD), a key enzyme of the oxidative pentose phosphate pathway. The conservation of the cryptic PTS2 motif suggests a biological function. We observed that translation from a non-AUG start codon generates an N-terminally extended peroxisomal isoform of Ustilago maydis PGD. Non-canonical initiation occurred at the sequence AGG AUU, consisting of two near-cognate start codons in tandem. Taken together, our data reveal non-AUG translation initiation as an additional mechanism to achieve the dual localization of a protein required both in the cytosol and the peroxisomes. Frontiers Media S.A. 2020-05-05 /pmc/articles/PMC7214817/ /pubmed/32432107 http://dx.doi.org/10.3389/fcell.2020.00251 Text en Copyright © 2020 Kremp, Bittner, Martorana, Klingenberger, Stehlik, Bölker and Freitag. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Kremp, Marco
Bittner, Elena
Martorana, Domenica
Klingenberger, Alexander
Stehlik, Thorsten
Bölker, Michael
Freitag, Johannes
Non-AUG Translation Initiation Generates Peroxisomal Isoforms of 6-Phosphogluconate Dehydrogenase in Fungi
title Non-AUG Translation Initiation Generates Peroxisomal Isoforms of 6-Phosphogluconate Dehydrogenase in Fungi
title_full Non-AUG Translation Initiation Generates Peroxisomal Isoforms of 6-Phosphogluconate Dehydrogenase in Fungi
title_fullStr Non-AUG Translation Initiation Generates Peroxisomal Isoforms of 6-Phosphogluconate Dehydrogenase in Fungi
title_full_unstemmed Non-AUG Translation Initiation Generates Peroxisomal Isoforms of 6-Phosphogluconate Dehydrogenase in Fungi
title_short Non-AUG Translation Initiation Generates Peroxisomal Isoforms of 6-Phosphogluconate Dehydrogenase in Fungi
title_sort non-aug translation initiation generates peroxisomal isoforms of 6-phosphogluconate dehydrogenase in fungi
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214817/
https://www.ncbi.nlm.nih.gov/pubmed/32432107
http://dx.doi.org/10.3389/fcell.2020.00251
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