GoldenBac: a simple, highly efficient, and widely applicable system for construction of multi-gene expression vectors for use with the baculovirus expression vector system

BACKGROUND: Recombinant protein production and purification of large protein complexes in eukaryotes requires efficient methods to generate multi-gene expression constructs, where each individual gene is under the control of its own promoter and terminator. Current methods are based either on serial...

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Detalles Bibliográficos
Autores principales: Neuhold, Jana, Radakovics, Katharina, Lehner, Anita, Weissmann, Florian, Garcia, Maria Queralt, Romero, Mari Carmen, Berrow, Nicholas S., Stolt-Bergner, Peggy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216392/
https://www.ncbi.nlm.nih.gov/pubmed/32398045
http://dx.doi.org/10.1186/s12896-020-00616-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216392/
https://www.ncbi.nlm.nih.gov/pubmed/32398045
http://dx.doi.org/10.1186/s12896-020-00616-z

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