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Differentiation of skeletal muscle Mesenchymal progenitor cells to myofibroblasts is reversible

Accumulation of intramuscular adipose tissue (IMAT) and development of fibrous tissues due to accumulation of collagen both affect meat quality such as tenderness, texture, and flavor. Thus, it is important for the production of high‐quality meat to regulate the amount of adipose and fibrous tissues...

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Autores principales: Takeuchi, Shiho, Yamanouchi, Keitaro, Sugihara, Hidetoshi, Matsuwaki, Takashi, Nishihara, Masugi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216888/
https://www.ncbi.nlm.nih.gov/pubmed/32285501
http://dx.doi.org/10.1111/asj.13368
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author Takeuchi, Shiho
Yamanouchi, Keitaro
Sugihara, Hidetoshi
Matsuwaki, Takashi
Nishihara, Masugi
author_facet Takeuchi, Shiho
Yamanouchi, Keitaro
Sugihara, Hidetoshi
Matsuwaki, Takashi
Nishihara, Masugi
author_sort Takeuchi, Shiho
collection PubMed
description Accumulation of intramuscular adipose tissue (IMAT) and development of fibrous tissues due to accumulation of collagen both affect meat quality such as tenderness, texture, and flavor. Thus, it is important for the production of high‐quality meat to regulate the amount of adipose and fibrous tissues in skeletal muscle. IMAT is comprised of adipocytes, while collagens included in fibrous tissues are mainly produced by activated fibroblasts. Both adipocytes and fibroblasts are differentiated from their common ancestors, called mesenchymal progenitor cells (MPC). We previously established rat MPC clone, 2G11 cells. As several reports implicated the plasticity of fibroblast differentiation, in the present study, using 2G11 cells, we asked whether myofibroblasts differentiated from MPC are capable of re‐gaining adipogenic potential in vitro. By treating with bFGF, their αSMA expression was reduced and adipogenic potential was restored partially. Furthermore, by lowering cell density together with bFGF treatment, 2G11 cell‐derived myofibroblasts lost αSMA expression and showed the highest adipogenic potential, and this was along with their morphological change from flattened‐ to spindle‐like shape, which is typically observed with MPC. These results indicated that MPC‐derived myofibroblasts could re‐acquire adipogenic potential, possibly mediated through returning to an undifferentiated MPC‐like state.
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spelling pubmed-72168882020-05-13 Differentiation of skeletal muscle Mesenchymal progenitor cells to myofibroblasts is reversible Takeuchi, Shiho Yamanouchi, Keitaro Sugihara, Hidetoshi Matsuwaki, Takashi Nishihara, Masugi Anim Sci J Original Articles Accumulation of intramuscular adipose tissue (IMAT) and development of fibrous tissues due to accumulation of collagen both affect meat quality such as tenderness, texture, and flavor. Thus, it is important for the production of high‐quality meat to regulate the amount of adipose and fibrous tissues in skeletal muscle. IMAT is comprised of adipocytes, while collagens included in fibrous tissues are mainly produced by activated fibroblasts. Both adipocytes and fibroblasts are differentiated from their common ancestors, called mesenchymal progenitor cells (MPC). We previously established rat MPC clone, 2G11 cells. As several reports implicated the plasticity of fibroblast differentiation, in the present study, using 2G11 cells, we asked whether myofibroblasts differentiated from MPC are capable of re‐gaining adipogenic potential in vitro. By treating with bFGF, their αSMA expression was reduced and adipogenic potential was restored partially. Furthermore, by lowering cell density together with bFGF treatment, 2G11 cell‐derived myofibroblasts lost αSMA expression and showed the highest adipogenic potential, and this was along with their morphological change from flattened‐ to spindle‐like shape, which is typically observed with MPC. These results indicated that MPC‐derived myofibroblasts could re‐acquire adipogenic potential, possibly mediated through returning to an undifferentiated MPC‐like state. John Wiley and Sons Inc. 2020-04-13 2020 /pmc/articles/PMC7216888/ /pubmed/32285501 http://dx.doi.org/10.1111/asj.13368 Text en © 2020 The Authors. Animal Science Journal published by John Wiley & Sons Australia, Ltd on behalf of Japanese Society of Animal Science This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Takeuchi, Shiho
Yamanouchi, Keitaro
Sugihara, Hidetoshi
Matsuwaki, Takashi
Nishihara, Masugi
Differentiation of skeletal muscle Mesenchymal progenitor cells to myofibroblasts is reversible
title Differentiation of skeletal muscle Mesenchymal progenitor cells to myofibroblasts is reversible
title_full Differentiation of skeletal muscle Mesenchymal progenitor cells to myofibroblasts is reversible
title_fullStr Differentiation of skeletal muscle Mesenchymal progenitor cells to myofibroblasts is reversible
title_full_unstemmed Differentiation of skeletal muscle Mesenchymal progenitor cells to myofibroblasts is reversible
title_short Differentiation of skeletal muscle Mesenchymal progenitor cells to myofibroblasts is reversible
title_sort differentiation of skeletal muscle mesenchymal progenitor cells to myofibroblasts is reversible
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216888/
https://www.ncbi.nlm.nih.gov/pubmed/32285501
http://dx.doi.org/10.1111/asj.13368
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