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An advanced cell cycle tag toolbox reveals principles underlying temporal control of structure-selective nucleases

Cell cycle tags allow to restrict target protein expression to specific cell cycle phases. Here, we present an advanced toolbox of cell cycle tag constructs in budding yeast with defined and compatible peak expression that allow comparison of protein functionality at different cell cycle phases. We...

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Detalles Bibliográficos
Autores principales: Bittmann, Julia, Grigaitis, Rokas, Galanti, Lorenzo, Amarell, Silas, Wilfling, Florian, Matos, Joao, Pfander, Boris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220381/
https://www.ncbi.nlm.nih.gov/pubmed/32352375
http://dx.doi.org/10.7554/eLife.52459