Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification

Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates. rTBL-1 was expressed in Pichia pastoris, yielding 316 mg per liter of culture, and was purified by nickel affinity ch...

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Detalles Bibliográficos
Autores principales: Martínez-Alarcón, Dania, Varrot, Annabelle, Fitches, Elaine, Gatehouse, John A., Cao, Min, Pyati, Prashant, Blanco-Labra, Alejandro, Garcia-Gasca, Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226325/
https://www.ncbi.nlm.nih.gov/pubmed/32340396
http://dx.doi.org/10.3390/biom10040654

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226325/
https://www.ncbi.nlm.nih.gov/pubmed/32340396
http://dx.doi.org/10.3390/biom10040654

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