High efficiency CHO cell display-based antibody maturation

Previously, we developed a CHO cell display-based antibody maturation procedure in which an antibody (or other protein) gene of interest was induced to mutate by activation-induced cytidine deaminase (AID) and then form a library by simply proliferating the CHO cells in culture. In this study, we fu...

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Autores principales: Luo, Ruiqi, Zhao, Yun, Fan, Yingjun, An, Lili, Jiang, Tao, Ma, Shaohua, Hang, Haiying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229201/
https://www.ncbi.nlm.nih.gov/pubmed/32415149
http://dx.doi.org/10.1038/s41598-020-65044-7
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author Luo, Ruiqi
Zhao, Yun
Fan, Yingjun
An, Lili
Jiang, Tao
Ma, Shaohua
Hang, Haiying
author_facet Luo, Ruiqi
Zhao, Yun
Fan, Yingjun
An, Lili
Jiang, Tao
Ma, Shaohua
Hang, Haiying
author_sort Luo, Ruiqi
collection PubMed
description Previously, we developed a CHO cell display-based antibody maturation procedure in which an antibody (or other protein) gene of interest was induced to mutate by activation-induced cytidine deaminase (AID) and then form a library by simply proliferating the CHO cells in culture. In this study, we further improved the efficiency of this maturation system by reengineering AID, and optimizing the nucleic acid sequence of the target antibody gene and AID gene as well as the protocol for AID gene transfection. These changes have increased both the mutation rate and the number of mutation type of antibody genes by more than 10 fold, and greatly improved the maturation efficiency of antibody/other proteins.
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spelling pubmed-72292012020-05-26 High efficiency CHO cell display-based antibody maturation Luo, Ruiqi Zhao, Yun Fan, Yingjun An, Lili Jiang, Tao Ma, Shaohua Hang, Haiying Sci Rep Article Previously, we developed a CHO cell display-based antibody maturation procedure in which an antibody (or other protein) gene of interest was induced to mutate by activation-induced cytidine deaminase (AID) and then form a library by simply proliferating the CHO cells in culture. In this study, we further improved the efficiency of this maturation system by reengineering AID, and optimizing the nucleic acid sequence of the target antibody gene and AID gene as well as the protocol for AID gene transfection. These changes have increased both the mutation rate and the number of mutation type of antibody genes by more than 10 fold, and greatly improved the maturation efficiency of antibody/other proteins. Nature Publishing Group UK 2020-05-15 /pmc/articles/PMC7229201/ /pubmed/32415149 http://dx.doi.org/10.1038/s41598-020-65044-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Luo, Ruiqi
Zhao, Yun
Fan, Yingjun
An, Lili
Jiang, Tao
Ma, Shaohua
Hang, Haiying
High efficiency CHO cell display-based antibody maturation
title High efficiency CHO cell display-based antibody maturation
title_full High efficiency CHO cell display-based antibody maturation
title_fullStr High efficiency CHO cell display-based antibody maturation
title_full_unstemmed High efficiency CHO cell display-based antibody maturation
title_short High efficiency CHO cell display-based antibody maturation
title_sort high efficiency cho cell display-based antibody maturation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229201/
https://www.ncbi.nlm.nih.gov/pubmed/32415149
http://dx.doi.org/10.1038/s41598-020-65044-7
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