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Visualization of spatial gene expression in plants by modified RNAscope fluorescent in situ hybridization

BACKGROUND: In situ analysis of biomarkers such as DNA, RNA and proteins are important for research and diagnostic purposes. At the RNA level, plant gene expression studies rely on qPCR, RNAseq and probe-based in situ hybridization (ISH). However, for ISH experiments poor stability of RNA and RNA ba...

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Detalles Bibliográficos
Autores principales: Solanki, Shyam, Ameen, Gazala, Zhao, Jin, Flaten, Jordan, Borowicz, Pawel, Brueggeman, Robert S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229616/
https://www.ncbi.nlm.nih.gov/pubmed/32467719
http://dx.doi.org/10.1186/s13007-020-00614-4