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K6-linked SUMOylation of BAF regulates nuclear integrity and DNA replication in mammalian cells

Barrier-to-autointegration factor (BAF) is a highly conserved protein in metazoans that has multiple functions during the cell cycle. We found that BAF is SUMOylated at K6, and that this modification is essential for its nuclear localization and function, including nuclear integrity maintenance and...

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Autores principales: Lin, Qiaoyu, Yu, Bin, Wang, Xiangyang, Zhu, Shicong, Zhao, Gan, Jia, Mingkang, Huang, Fan, Xu, Nan, Ren, He, Jiang, Qing, Zhang, Chuanmao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229763/
https://www.ncbi.nlm.nih.gov/pubmed/32332162
http://dx.doi.org/10.1073/pnas.1912984117
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author Lin, Qiaoyu
Yu, Bin
Wang, Xiangyang
Zhu, Shicong
Zhao, Gan
Jia, Mingkang
Huang, Fan
Xu, Nan
Ren, He
Jiang, Qing
Zhang, Chuanmao
author_facet Lin, Qiaoyu
Yu, Bin
Wang, Xiangyang
Zhu, Shicong
Zhao, Gan
Jia, Mingkang
Huang, Fan
Xu, Nan
Ren, He
Jiang, Qing
Zhang, Chuanmao
author_sort Lin, Qiaoyu
collection PubMed
description Barrier-to-autointegration factor (BAF) is a highly conserved protein in metazoans that has multiple functions during the cell cycle. We found that BAF is SUMOylated at K6, and that this modification is essential for its nuclear localization and function, including nuclear integrity maintenance and DNA replication. K6-linked SUMOylation of BAF promotes binding and interaction with lamin A/C to regulate nuclear integrity. K6-linked SUMOylation of BAF also supports BAF binding to DNA and proliferating cell nuclear antigen and regulates DNA replication. SENP1 and SENP2 catalyze the de-SUMOylation of BAF at K6. Disrupting the SUMOylation and de-SUMOylation cycle of BAF at K6 not only disturbs nuclear integrity, but also induces DNA replication failure. Taken together, our findings demonstrate that SUMOylation at K6 is an important regulatory mechanism that governs the nuclear functions of BAF in mammalian cells.
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spelling pubmed-72297632020-05-26 K6-linked SUMOylation of BAF regulates nuclear integrity and DNA replication in mammalian cells Lin, Qiaoyu Yu, Bin Wang, Xiangyang Zhu, Shicong Zhao, Gan Jia, Mingkang Huang, Fan Xu, Nan Ren, He Jiang, Qing Zhang, Chuanmao Proc Natl Acad Sci U S A Biological Sciences Barrier-to-autointegration factor (BAF) is a highly conserved protein in metazoans that has multiple functions during the cell cycle. We found that BAF is SUMOylated at K6, and that this modification is essential for its nuclear localization and function, including nuclear integrity maintenance and DNA replication. K6-linked SUMOylation of BAF promotes binding and interaction with lamin A/C to regulate nuclear integrity. K6-linked SUMOylation of BAF also supports BAF binding to DNA and proliferating cell nuclear antigen and regulates DNA replication. SENP1 and SENP2 catalyze the de-SUMOylation of BAF at K6. Disrupting the SUMOylation and de-SUMOylation cycle of BAF at K6 not only disturbs nuclear integrity, but also induces DNA replication failure. Taken together, our findings demonstrate that SUMOylation at K6 is an important regulatory mechanism that governs the nuclear functions of BAF in mammalian cells. National Academy of Sciences 2020-05-12 2020-04-24 /pmc/articles/PMC7229763/ /pubmed/32332162 http://dx.doi.org/10.1073/pnas.1912984117 Text en Copyright © 2020 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Lin, Qiaoyu
Yu, Bin
Wang, Xiangyang
Zhu, Shicong
Zhao, Gan
Jia, Mingkang
Huang, Fan
Xu, Nan
Ren, He
Jiang, Qing
Zhang, Chuanmao
K6-linked SUMOylation of BAF regulates nuclear integrity and DNA replication in mammalian cells
title K6-linked SUMOylation of BAF regulates nuclear integrity and DNA replication in mammalian cells
title_full K6-linked SUMOylation of BAF regulates nuclear integrity and DNA replication in mammalian cells
title_fullStr K6-linked SUMOylation of BAF regulates nuclear integrity and DNA replication in mammalian cells
title_full_unstemmed K6-linked SUMOylation of BAF regulates nuclear integrity and DNA replication in mammalian cells
title_short K6-linked SUMOylation of BAF regulates nuclear integrity and DNA replication in mammalian cells
title_sort k6-linked sumoylation of baf regulates nuclear integrity and dna replication in mammalian cells
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229763/
https://www.ncbi.nlm.nih.gov/pubmed/32332162
http://dx.doi.org/10.1073/pnas.1912984117
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