Preferential production of RNA rings by T4 RNA ligase 2 without any splint through rational design of precursor strand

Rings of single-stranded RNA are promising for many practical applications, but the methods to prepare them in preparative scale have never been established. Previously, RNA circularization was achieved by T4 RNA ligase 2 (Rnl2, a dsRNA ligase) using splints, but the yield was low due to concurrent...

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Detalles Bibliográficos
Autores principales: Chen, Hui, Cheng, Kai, Liu, Xiaoli, An, Ran, Komiyama, Makoto, Liang, Xingguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229815/
https://www.ncbi.nlm.nih.gov/pubmed/32232357
http://dx.doi.org/10.1093/nar/gkaa181

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229815/
https://www.ncbi.nlm.nih.gov/pubmed/32232357
http://dx.doi.org/10.1093/nar/gkaa181

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