Cargando…
Preferential production of RNA rings by T4 RNA ligase 2 without any splint through rational design of precursor strand
Rings of single-stranded RNA are promising for many practical applications, but the methods to prepare them in preparative scale have never been established. Previously, RNA circularization was achieved by T4 RNA ligase 2 (Rnl2, a dsRNA ligase) using splints, but the yield was low due to concurrent...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2020
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229815/ https://www.ncbi.nlm.nih.gov/pubmed/32232357 http://dx.doi.org/10.1093/nar/gkaa181 |