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Amino Acid Composition of Novel Plant Drinks from Oat, Lentil and Pea

Plant-based drinks (PBDs) as alternatives to milk is a fast-growing market in much of the western world, with the demand increasing every year. However, most PBDs from a single plant ingredient do not have an amino acid profile that matches human needs. Therefore, this study set out to combine plant...

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Autores principales: Bonke, Astrid, Sieuwerts, Sander, Petersen, Iben Lykke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230155/
https://www.ncbi.nlm.nih.gov/pubmed/32260282
http://dx.doi.org/10.3390/foods9040429
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author Bonke, Astrid
Sieuwerts, Sander
Petersen, Iben Lykke
author_facet Bonke, Astrid
Sieuwerts, Sander
Petersen, Iben Lykke
author_sort Bonke, Astrid
collection PubMed
description Plant-based drinks (PBDs) as alternatives to milk is a fast-growing market in much of the western world, with the demand increasing every year. However, most PBDs from a single plant ingredient do not have an amino acid profile that matches human needs. Therefore, this study set out to combine plant ingredients to achieve a more balanced amino acid profile of novel plant drinks, by combining a high content of oat with the pulses pea (Pisum sativum) and lentil (Lens culinaris) in a solution. After removal of the sediment, the resulting plant drinks were composed of what could be kept in suspension. The amino acid and protein composition of the plant drinks were investigated with capillary electrophoresis, to identify the amino acids, and SDS-PAGE to assess the proteins present. The amino acid profile was compared against recommended daily intake (RDI). It was determined that the plant drinks with only oat and lentil did not have a strong amino acid profile, likely due to the higher pH of the lentil concentrate affecting which proteins could be kept in solution. Plant drinks with a combination of both lentil and pea, or only pea, added to the oat drink had an improved concentration of the amino acids that were otherwise in the low end compared to RDI. This includes a high content of phenylalanine, leucine and threonine, as well as a moderate amount of isoleucine, valine and methionine, and a contribution of histidine and lysine. An assessment of stability and sensory parameters was also conducted, concluding there was an advantage of combining oat with a legume, especially pea.
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spelling pubmed-72301552020-05-28 Amino Acid Composition of Novel Plant Drinks from Oat, Lentil and Pea Bonke, Astrid Sieuwerts, Sander Petersen, Iben Lykke Foods Article Plant-based drinks (PBDs) as alternatives to milk is a fast-growing market in much of the western world, with the demand increasing every year. However, most PBDs from a single plant ingredient do not have an amino acid profile that matches human needs. Therefore, this study set out to combine plant ingredients to achieve a more balanced amino acid profile of novel plant drinks, by combining a high content of oat with the pulses pea (Pisum sativum) and lentil (Lens culinaris) in a solution. After removal of the sediment, the resulting plant drinks were composed of what could be kept in suspension. The amino acid and protein composition of the plant drinks were investigated with capillary electrophoresis, to identify the amino acids, and SDS-PAGE to assess the proteins present. The amino acid profile was compared against recommended daily intake (RDI). It was determined that the plant drinks with only oat and lentil did not have a strong amino acid profile, likely due to the higher pH of the lentil concentrate affecting which proteins could be kept in solution. Plant drinks with a combination of both lentil and pea, or only pea, added to the oat drink had an improved concentration of the amino acids that were otherwise in the low end compared to RDI. This includes a high content of phenylalanine, leucine and threonine, as well as a moderate amount of isoleucine, valine and methionine, and a contribution of histidine and lysine. An assessment of stability and sensory parameters was also conducted, concluding there was an advantage of combining oat with a legume, especially pea. MDPI 2020-04-03 /pmc/articles/PMC7230155/ /pubmed/32260282 http://dx.doi.org/10.3390/foods9040429 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bonke, Astrid
Sieuwerts, Sander
Petersen, Iben Lykke
Amino Acid Composition of Novel Plant Drinks from Oat, Lentil and Pea
title Amino Acid Composition of Novel Plant Drinks from Oat, Lentil and Pea
title_full Amino Acid Composition of Novel Plant Drinks from Oat, Lentil and Pea
title_fullStr Amino Acid Composition of Novel Plant Drinks from Oat, Lentil and Pea
title_full_unstemmed Amino Acid Composition of Novel Plant Drinks from Oat, Lentil and Pea
title_short Amino Acid Composition of Novel Plant Drinks from Oat, Lentil and Pea
title_sort amino acid composition of novel plant drinks from oat, lentil and pea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230155/
https://www.ncbi.nlm.nih.gov/pubmed/32260282
http://dx.doi.org/10.3390/foods9040429
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