A Gold Standard, CRISPR/Cas9-Based Complementation Strategy Reliant on 24 Nucleotide Bookmark Sequences

Phenotypic complementation of gene knockouts is an essential step in establishing function. Here, we describe a simple strategy for ‘gold standard’ complementation in which the mutant allele is replaced in situ with a wild type (WT) allele in a procedure that exploits CRISPR/Cas9. The method relies...

Descripción completa

Detalles Bibliográficos
Autores principales: Seys, François M., Rowe, Peter, Bolt, Edward L., Humphreys, Christopher M., Minton, Nigel P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Concept Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230483/
https://www.ncbi.nlm.nih.gov/pubmed/32340238
http://dx.doi.org/10.3390/genes11040458

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230483/
https://www.ncbi.nlm.nih.gov/pubmed/32340238
http://dx.doi.org/10.3390/genes11040458

Ejemplares similares