Cargando…
Setup of an Extraction Method for the Analysis of Carotenoids in Microgreens
Microgreens are gaining increasing interest as a potential functional food due to their relevant contents of micronutrients and bioactive compounds, including carotenoids. Nevertheless, the analysis of carotenoids is inherently difficult, due to their thermal and chemical susceptibility, as well as...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231143/ https://www.ncbi.nlm.nih.gov/pubmed/32276423 http://dx.doi.org/10.3390/foods9040459 |
_version_ | 1783535122976342016 |
---|---|
author | Paradiso, Vito Michele Castellino, Maria Renna, Massimiliano Santamaria, Pietro Caponio, Francesco |
author_facet | Paradiso, Vito Michele Castellino, Maria Renna, Massimiliano Santamaria, Pietro Caponio, Francesco |
author_sort | Paradiso, Vito Michele |
collection | PubMed |
description | Microgreens are gaining increasing interest as a potential functional food due to their relevant contents of micronutrients and bioactive compounds, including carotenoids. Nevertheless, the analysis of carotenoids is inherently difficult, due to their thermal and chemical susceptibility, as well as to their varying polarity. From this point of view, extraction is the most critical step, compared to chromatographic separation and detection. Thus, the reliability of data on carotenoids should be guaranteed by a constant focus on analytical issues, with appropriate adaptations to each sample matrix. In this research, a specific extraction procedure for the analysis of carotenoids in microgreens was developed. Solvent composition, extraction time, solvent/sample ratio, and repeated extractions were evaluated. The obtained protocol showed recovery of 97.2%, limits of quantitation of 5.2 μg·g(−1) for lutein and 15.9 μg·g(−1) for β-carotene, as well as intra-day mean repeatability of 5.7% and inter-day mean repeatability of 4.7%. |
format | Online Article Text |
id | pubmed-7231143 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72311432020-05-22 Setup of an Extraction Method for the Analysis of Carotenoids in Microgreens Paradiso, Vito Michele Castellino, Maria Renna, Massimiliano Santamaria, Pietro Caponio, Francesco Foods Article Microgreens are gaining increasing interest as a potential functional food due to their relevant contents of micronutrients and bioactive compounds, including carotenoids. Nevertheless, the analysis of carotenoids is inherently difficult, due to their thermal and chemical susceptibility, as well as to their varying polarity. From this point of view, extraction is the most critical step, compared to chromatographic separation and detection. Thus, the reliability of data on carotenoids should be guaranteed by a constant focus on analytical issues, with appropriate adaptations to each sample matrix. In this research, a specific extraction procedure for the analysis of carotenoids in microgreens was developed. Solvent composition, extraction time, solvent/sample ratio, and repeated extractions were evaluated. The obtained protocol showed recovery of 97.2%, limits of quantitation of 5.2 μg·g(−1) for lutein and 15.9 μg·g(−1) for β-carotene, as well as intra-day mean repeatability of 5.7% and inter-day mean repeatability of 4.7%. MDPI 2020-04-08 /pmc/articles/PMC7231143/ /pubmed/32276423 http://dx.doi.org/10.3390/foods9040459 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Paradiso, Vito Michele Castellino, Maria Renna, Massimiliano Santamaria, Pietro Caponio, Francesco Setup of an Extraction Method for the Analysis of Carotenoids in Microgreens |
title | Setup of an Extraction Method for the Analysis of Carotenoids in Microgreens |
title_full | Setup of an Extraction Method for the Analysis of Carotenoids in Microgreens |
title_fullStr | Setup of an Extraction Method for the Analysis of Carotenoids in Microgreens |
title_full_unstemmed | Setup of an Extraction Method for the Analysis of Carotenoids in Microgreens |
title_short | Setup of an Extraction Method for the Analysis of Carotenoids in Microgreens |
title_sort | setup of an extraction method for the analysis of carotenoids in microgreens |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231143/ https://www.ncbi.nlm.nih.gov/pubmed/32276423 http://dx.doi.org/10.3390/foods9040459 |
work_keys_str_mv | AT paradisovitomichele setupofanextractionmethodfortheanalysisofcarotenoidsinmicrogreens AT castellinomaria setupofanextractionmethodfortheanalysisofcarotenoidsinmicrogreens AT rennamassimiliano setupofanextractionmethodfortheanalysisofcarotenoidsinmicrogreens AT santamariapietro setupofanextractionmethodfortheanalysisofcarotenoidsinmicrogreens AT caponiofrancesco setupofanextractionmethodfortheanalysisofcarotenoidsinmicrogreens |