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Change of Amino Acid Residues in Idiotypic Nanobodies Enhanced the Sensitivity of Competitive Enzyme Immunoassay for Mycotoxin Ochratoxin A in Cereals

Anti-idiotypic nanobodies, usually expressed by gene engineering protocol, has been shown as a nontoxic coating antigen for toxic compound immunoassays. We here focused on how to increase immunoassay sensitivity by changing the nanobody’s primary sequence. In the experiments, two anti-idiotype nanob...

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Detalles Bibliográficos
Autores principales: Zhang, Caixia, Zhang, Weiqi, Tang, Xiaoqian, Zhang, Qi, Zhang, Wen, Li, Peiwu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232238/
https://www.ncbi.nlm.nih.gov/pubmed/32340239
http://dx.doi.org/10.3390/toxins12040273