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Improved Protocol for DNA Extraction from Subsoils Using Phosphate Lysis Buffer

As our understanding of soil biology deepens, there is a growing demand for investigations addressing microbial processes in the earth beneath the topsoil layer, called subsoil. High clay content in subsoils often hinders the recovery of sufficient quantities of DNA as clay particles bind nucleic ac...

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Detalles Bibliográficos
Autores principales: Guerra, Victor, Beule, Lukas, Lehtsaar, Ena, Liao, Hui-Ling, Karlovsky, Petr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232467/
https://www.ncbi.nlm.nih.gov/pubmed/32272709
http://dx.doi.org/10.3390/microorganisms8040532