GoPrime: Development of an In Silico Framework to Predict the Performance of Real-Time PCR Primers and Probes Using Foot-and-Mouth Disease Virus as a Model

Real-time PCR (rPCR) is a widely accepted diagnostic tool for the detection and quantification of nucleic acid targets. In order for these assays to achieve high sensitivity and specificity, primer and probe-template complementarity is essential; however, mismatches are often unavoidable and can res...

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Detalles Bibliográficos
Autores principales: Howson, Emma L A, Orton, Richard J, Mioulet, Valerie, Lembo, Tiziana, King, Donald P, Fowler, Veronica L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238122/
https://www.ncbi.nlm.nih.gov/pubmed/32326039
http://dx.doi.org/10.3390/pathogens9040303

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238122/
https://www.ncbi.nlm.nih.gov/pubmed/32326039
http://dx.doi.org/10.3390/pathogens9040303

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