Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses

Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-ß (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sens...

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Autores principales: Prasanth, K. Reddisiva, Hirano, Minato, Fagg, W. Samuel, McAnarney, Eileen T., Shan, Chao, Xie, Xuping, Hage, Adam, Pietzsch, Colette A., Bukreyev, Alexander, Rajsbaum, Ricardo, Shi, Pei-Yong, Bedford, Mark T., Bradrick, Shelton S., Menachery, Vineet, Garcia-Blanco, Mariano A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239047/
https://www.ncbi.nlm.nih.gov/pubmed/32511318
http://dx.doi.org/10.1101/2020.03.24.005900
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author Prasanth, K. Reddisiva
Hirano, Minato
Fagg, W. Samuel
McAnarney, Eileen T.
Shan, Chao
Xie, Xuping
Hage, Adam
Pietzsch, Colette A.
Bukreyev, Alexander
Rajsbaum, Ricardo
Shi, Pei-Yong
Bedford, Mark T.
Bradrick, Shelton S.
Menachery, Vineet
Garcia-Blanco, Mariano A.
author_facet Prasanth, K. Reddisiva
Hirano, Minato
Fagg, W. Samuel
McAnarney, Eileen T.
Shan, Chao
Xie, Xuping
Hage, Adam
Pietzsch, Colette A.
Bukreyev, Alexander
Rajsbaum, Ricardo
Shi, Pei-Yong
Bedford, Mark T.
Bradrick, Shelton S.
Menachery, Vineet
Garcia-Blanco, Mariano A.
author_sort Prasanth, K. Reddisiva
collection PubMed
description Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-ß (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.
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spelling pubmed-72390472020-06-07 Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses Prasanth, K. Reddisiva Hirano, Minato Fagg, W. Samuel McAnarney, Eileen T. Shan, Chao Xie, Xuping Hage, Adam Pietzsch, Colette A. Bukreyev, Alexander Rajsbaum, Ricardo Shi, Pei-Yong Bedford, Mark T. Bradrick, Shelton S. Menachery, Vineet Garcia-Blanco, Mariano A. bioRxiv Article Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-ß (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target. Cold Spring Harbor Laboratory 2020-03-27 /pmc/articles/PMC7239047/ /pubmed/32511318 http://dx.doi.org/10.1101/2020.03.24.005900 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/It is made available under a CC-BY-NC-ND 4.0 International license (http://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Article
Prasanth, K. Reddisiva
Hirano, Minato
Fagg, W. Samuel
McAnarney, Eileen T.
Shan, Chao
Xie, Xuping
Hage, Adam
Pietzsch, Colette A.
Bukreyev, Alexander
Rajsbaum, Ricardo
Shi, Pei-Yong
Bedford, Mark T.
Bradrick, Shelton S.
Menachery, Vineet
Garcia-Blanco, Mariano A.
Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses
title Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses
title_full Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses
title_fullStr Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses
title_full_unstemmed Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses
title_short Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses
title_sort topoisomerase iii-ß is required for efficient replication of positive-sense rna viruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239047/
https://www.ncbi.nlm.nih.gov/pubmed/32511318
http://dx.doi.org/10.1101/2020.03.24.005900
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