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Small heat shock proteins determine synapse number and neuronal activity during development

Environmental changes cause stress, Reactive Oxygen Species and unfolded protein accumulation which hamper synaptic activity and trigger cell death. Heat shock proteins (HSPs) assist protein refolding to maintain proteostasis and cellular integrity. Mechanisms regulating the activity of HSPs include...

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Detalles Bibliográficos
Autores principales: Santana, Elena, de los Reyes, Teresa, Casas-Tintó, Sergio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241713/
https://www.ncbi.nlm.nih.gov/pubmed/32437379
http://dx.doi.org/10.1371/journal.pone.0233231
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author Santana, Elena
de los Reyes, Teresa
Casas-Tintó, Sergio
author_facet Santana, Elena
de los Reyes, Teresa
Casas-Tintó, Sergio
author_sort Santana, Elena
collection PubMed
description Environmental changes cause stress, Reactive Oxygen Species and unfolded protein accumulation which hamper synaptic activity and trigger cell death. Heat shock proteins (HSPs) assist protein refolding to maintain proteostasis and cellular integrity. Mechanisms regulating the activity of HSPs include transcription factors and posttranslational modifications that ensure a rapid response. HSPs preserve synaptic function in the nervous system upon environmental insults or pathological factors and contribute to the coupling between environmental cues and neuron control of development. We have performed a biased screening in Drosophila melanogaster searching for synaptogenic modulators among HSPs during development. We explore the role of two small-HSPs (sHSPs), sHSP23 and sHSP26 in synaptogenesis and neuronal activity. Both sHSPs immunoprecipitate together and the equilibrium between both chaperones is required for neuronal development and activity. The molecular mechanism controlling HSP23 and HSP26 accumulation in neurons relies on a novel gene (CG1561), which we name Pinkman (pkm). We propose that sHSPs and Pkm are targets to modulate the impact of stress in neurons and to prevent synapse loss.
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spelling pubmed-72417132020-06-08 Small heat shock proteins determine synapse number and neuronal activity during development Santana, Elena de los Reyes, Teresa Casas-Tintó, Sergio PLoS One Research Article Environmental changes cause stress, Reactive Oxygen Species and unfolded protein accumulation which hamper synaptic activity and trigger cell death. Heat shock proteins (HSPs) assist protein refolding to maintain proteostasis and cellular integrity. Mechanisms regulating the activity of HSPs include transcription factors and posttranslational modifications that ensure a rapid response. HSPs preserve synaptic function in the nervous system upon environmental insults or pathological factors and contribute to the coupling between environmental cues and neuron control of development. We have performed a biased screening in Drosophila melanogaster searching for synaptogenic modulators among HSPs during development. We explore the role of two small-HSPs (sHSPs), sHSP23 and sHSP26 in synaptogenesis and neuronal activity. Both sHSPs immunoprecipitate together and the equilibrium between both chaperones is required for neuronal development and activity. The molecular mechanism controlling HSP23 and HSP26 accumulation in neurons relies on a novel gene (CG1561), which we name Pinkman (pkm). We propose that sHSPs and Pkm are targets to modulate the impact of stress in neurons and to prevent synapse loss. Public Library of Science 2020-05-21 /pmc/articles/PMC7241713/ /pubmed/32437379 http://dx.doi.org/10.1371/journal.pone.0233231 Text en © 2020 Santana et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Santana, Elena
de los Reyes, Teresa
Casas-Tintó, Sergio
Small heat shock proteins determine synapse number and neuronal activity during development
title Small heat shock proteins determine synapse number and neuronal activity during development
title_full Small heat shock proteins determine synapse number and neuronal activity during development
title_fullStr Small heat shock proteins determine synapse number and neuronal activity during development
title_full_unstemmed Small heat shock proteins determine synapse number and neuronal activity during development
title_short Small heat shock proteins determine synapse number and neuronal activity during development
title_sort small heat shock proteins determine synapse number and neuronal activity during development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241713/
https://www.ncbi.nlm.nih.gov/pubmed/32437379
http://dx.doi.org/10.1371/journal.pone.0233231
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