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Selection and validation of reference genes for normalisation of gene expression in ischaemic and toxicological studies in kidney disease
Normalisation to standard reference gene(s) is essential for quantitative real-time polymerase chain reaction (RT-qPCR) to obtain reproducible and comparable results of a gene of interest (GOI) between subjects and under varying experimental conditions. There is limited evidence to support selection...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241806/ https://www.ncbi.nlm.nih.gov/pubmed/32437461 http://dx.doi.org/10.1371/journal.pone.0233109 |