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Selection and validation of reference genes for normalisation of gene expression in ischaemic and toxicological studies in kidney disease

Normalisation to standard reference gene(s) is essential for quantitative real-time polymerase chain reaction (RT-qPCR) to obtain reproducible and comparable results of a gene of interest (GOI) between subjects and under varying experimental conditions. There is limited evidence to support selection...

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Detalles Bibliográficos
Autores principales: Herath, Sanjeeva, Dai, Hongying, Erlich, Jonathan, Au, Amy YM, Taylor, Kylie, Succar, Lena, Endre, Zoltán H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241806/
https://www.ncbi.nlm.nih.gov/pubmed/32437461
http://dx.doi.org/10.1371/journal.pone.0233109