Fine-tuning the regulation of Cas9 expression levels for efficient CRISPR-Cas9 mediated recombination in Streptomyces

CRISPR-Cas9 has proven as a very powerful gene editing tool for Actinomyces, allowing scarless and precise genome editing in selected strains of these biotechnologically relevant microorganisms. However, its general application in actinomycetes has been limited due to its inefficacy when applying th...

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Detalles Bibliográficos
Autores principales: Ye, Suhui, Enghiad, Behnam, Zhao, Huimin, Takano, Eriko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2020
Materias:
Metabolic Engineering and Synthetic Biology - Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7244461/
https://www.ncbi.nlm.nih.gov/pubmed/32367443
http://dx.doi.org/10.1007/s10295-020-02277-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7244461/
https://www.ncbi.nlm.nih.gov/pubmed/32367443
http://dx.doi.org/10.1007/s10295-020-02277-5

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