Efficient dual-negative selection for bacterial genome editing

BACKGROUND: Gene editing is key for elucidating gene function. Traditional methods, such as consecutive single-crossovers, have been widely used to modify bacterial genomes. However, cumbersome cloning and limited efficiency of negative selection often make this method slower than other methods such...

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Detalles Bibliográficos
Autores principales: Cianfanelli, Francesca Romana, Cunrath, Olivier, Bumann, Dirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245781/
https://www.ncbi.nlm.nih.gov/pubmed/32448155
http://dx.doi.org/10.1186/s12866-020-01819-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245781/
https://www.ncbi.nlm.nih.gov/pubmed/32448155
http://dx.doi.org/10.1186/s12866-020-01819-2

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