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Efficient dual-negative selection for bacterial genome editing
BACKGROUND: Gene editing is key for elucidating gene function. Traditional methods, such as consecutive single-crossovers, have been widely used to modify bacterial genomes. However, cumbersome cloning and limited efficiency of negative selection often make this method slower than other methods such...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245781/ https://www.ncbi.nlm.nih.gov/pubmed/32448155 http://dx.doi.org/10.1186/s12866-020-01819-2 |