Homology-dependent recombination of large synthetic pathways into E. coli genome via λ-Red and CRISPR/Cas9 dependent selection methodology

BACKGROUND: Metabolic engineering frequently needs genomic integration of many heterologous genes for biosynthetic pathway assembly. Despite great progresses in genome editing for the model microorganism Escherichia coli, the integration of large pathway into genome for stabilized chemical productio...

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Detalles Bibliográficos
Autores principales: Su, Buli, Song, Dandan, Zhu, Honghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245811/
https://www.ncbi.nlm.nih.gov/pubmed/32448328
http://dx.doi.org/10.1186/s12934-020-01360-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245811/
https://www.ncbi.nlm.nih.gov/pubmed/32448328
http://dx.doi.org/10.1186/s12934-020-01360-x

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