Cargando…

Detection of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a toxicity bioindicator to the effects of nickel on Ni-Cr alloy prosthesis users

Previous studies have suggested that exposure to Ni from Ni-Cr alloys can affect the human body through oxidative stress. The present study discusses the effect of nickel from Ni-Cr alloy prostheses on the formation of DNA Adduct 8-Hydroxy-2′-Deoxyguanosine (8-OHdG), evaluated based on creatinine an...

Descripción completa

Detalles Bibliográficos
Autores principales: Utari, Dian Retno, Budiawan, Auerkari, Elza Ibrahim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253878/
https://www.ncbi.nlm.nih.gov/pubmed/32489306
http://dx.doi.org/10.1016/j.sjbs.2020.03.006
_version_ 1783539415747919872
author Utari, Dian Retno
Budiawan
Auerkari, Elza Ibrahim
author_facet Utari, Dian Retno
Budiawan
Auerkari, Elza Ibrahim
author_sort Utari, Dian Retno
collection PubMed
description Previous studies have suggested that exposure to Ni from Ni-Cr alloys can affect the human body through oxidative stress. The present study discusses the effect of nickel from Ni-Cr alloy prostheses on the formation of DNA Adduct 8-Hydroxy-2′-Deoxyguanosine (8-OHdG), evaluated based on creatinine and 8-OHdG concentrations in urine, determined with LC-MS/MS, for a Ni-Cr alloy user group and a never-user control group. The mean creatinine and 8-OHdG concentrations were not significantly different between the test groups, although highest levels were observed for the in the Ni-Cr user group. It is suggested that samples with relatively high creatinine and/or 8-OHdG levels are further studied in more detail for stability of concentrations and for the effect of contributing factors.
format Online
Article
Text
id pubmed-7253878
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-72538782020-06-01 Detection of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a toxicity bioindicator to the effects of nickel on Ni-Cr alloy prosthesis users Utari, Dian Retno Budiawan Auerkari, Elza Ibrahim Saudi J Biol Sci Article Previous studies have suggested that exposure to Ni from Ni-Cr alloys can affect the human body through oxidative stress. The present study discusses the effect of nickel from Ni-Cr alloy prostheses on the formation of DNA Adduct 8-Hydroxy-2′-Deoxyguanosine (8-OHdG), evaluated based on creatinine and 8-OHdG concentrations in urine, determined with LC-MS/MS, for a Ni-Cr alloy user group and a never-user control group. The mean creatinine and 8-OHdG concentrations were not significantly different between the test groups, although highest levels were observed for the in the Ni-Cr user group. It is suggested that samples with relatively high creatinine and/or 8-OHdG levels are further studied in more detail for stability of concentrations and for the effect of contributing factors. Elsevier 2020-06 2020-03-12 /pmc/articles/PMC7253878/ /pubmed/32489306 http://dx.doi.org/10.1016/j.sjbs.2020.03.006 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Utari, Dian Retno
Budiawan
Auerkari, Elza Ibrahim
Detection of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a toxicity bioindicator to the effects of nickel on Ni-Cr alloy prosthesis users
title Detection of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a toxicity bioindicator to the effects of nickel on Ni-Cr alloy prosthesis users
title_full Detection of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a toxicity bioindicator to the effects of nickel on Ni-Cr alloy prosthesis users
title_fullStr Detection of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a toxicity bioindicator to the effects of nickel on Ni-Cr alloy prosthesis users
title_full_unstemmed Detection of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a toxicity bioindicator to the effects of nickel on Ni-Cr alloy prosthesis users
title_short Detection of DNA adduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a toxicity bioindicator to the effects of nickel on Ni-Cr alloy prosthesis users
title_sort detection of dna adduct 8-hydroxy-2'-deoxyguanosine (8-ohdg) as a toxicity bioindicator to the effects of nickel on ni-cr alloy prosthesis users
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253878/
https://www.ncbi.nlm.nih.gov/pubmed/32489306
http://dx.doi.org/10.1016/j.sjbs.2020.03.006
work_keys_str_mv AT utaridianretno detectionofdnaadduct8hydroxy2deoxyguanosine8ohdgasatoxicitybioindicatortotheeffectsofnickelonnicralloyprosthesisusers
AT budiawan detectionofdnaadduct8hydroxy2deoxyguanosine8ohdgasatoxicitybioindicatortotheeffectsofnickelonnicralloyprosthesisusers
AT auerkarielzaibrahim detectionofdnaadduct8hydroxy2deoxyguanosine8ohdgasatoxicitybioindicatortotheeffectsofnickelonnicralloyprosthesisusers