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A non-fluorescent HaloTag blocker for improved measurement and visualization of protein synthesis in living cells
Background: HaloTag is a modified bacterial enzyme that binds rapidly and irreversibly to an array of synthetic ligands, including chemical dyes. When expressed in live cells in conjunction with a protein of interest, HaloTag can be used to study protein trafficking, synthesis, and degradation. For...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
F1000 Research Limited
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7255903/ https://www.ncbi.nlm.nih.gov/pubmed/32518633 http://dx.doi.org/10.12688/f1000research.23289.2 |