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Differentially Marked IncP-1β R751 Plasmids for Cloning via Recombineering and Conjugation
We demonstrate here for the first time the use of an IncP-1β plasmid, R751, as a gene capture vehicle for recombineering/conjugation strategies to clone large segments of bacterial genomes (20 – 100 + Kb). We designed R751 derivatives containing alternative markers for greater flexibility when using...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Exeley Inc.
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260700/ https://www.ncbi.nlm.nih.gov/pubmed/31880899 http://dx.doi.org/10.33073/pjm-2019-052 |