Caveat mutator: alanine substitutions for conserved amino acids in RNA ligase elicit unexpected rearrangements of the active site for lysine adenylylation

Naegleria gruberi RNA ligase (NgrRnl) exemplifies the Rnl5 family of adenosine triphosphate (ATP)-dependent polynucleotide ligases that seal 3′-OH RNA strands in the context of 3′-OH/5′-PO(4) nicked duplexes. Like all classic ligases, NgrRnl forms a covalent lysyl–AMP intermediate. A two-metal mecha...

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Detalles Bibliográficos
Autores principales: Unciuleac, Mihaela-Carmen, Goldgur, Yehuda, Shuman, Stewart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261155/
https://www.ncbi.nlm.nih.gov/pubmed/32315072
http://dx.doi.org/10.1093/nar/gkaa238

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261155/
https://www.ncbi.nlm.nih.gov/pubmed/32315072
http://dx.doi.org/10.1093/nar/gkaa238

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