TEX15 associates with MILI and silences transposable elements in male germ cells

DNA methylation is a major silencing mechanism of transposable elements (TEs). Here we report that TEX15, a testis-specific protein, is required for TE silencing. TEX15 is expressed in embryonic germ cells and functions during genome-wide epigenetic reprogramming. The Tex15 mutant exhibits DNA hypom...

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Autores principales: Yang, Fang, Lan, Yemin, Pandey, Radha Raman, Homolka, David, Berger, Shelley L., Pillai, Ramesh S., Bartolomei, Marisa S., Wang, P. Jeremy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2020
Materias:
Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263141/
https://www.ncbi.nlm.nih.gov/pubmed/32381626
http://dx.doi.org/10.1101/gad.335489.119
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author Yang, Fang
Lan, Yemin
Pandey, Radha Raman
Homolka, David
Berger, Shelley L.
Pillai, Ramesh S.
Bartolomei, Marisa S.
Wang, P. Jeremy
author_facet Yang, Fang
Lan, Yemin
Pandey, Radha Raman
Homolka, David
Berger, Shelley L.
Pillai, Ramesh S.
Bartolomei, Marisa S.
Wang, P. Jeremy
author_sort Yang, Fang
collection PubMed
description DNA methylation is a major silencing mechanism of transposable elements (TEs). Here we report that TEX15, a testis-specific protein, is required for TE silencing. TEX15 is expressed in embryonic germ cells and functions during genome-wide epigenetic reprogramming. The Tex15 mutant exhibits DNA hypomethylation in TEs at a level similar to Mili and Dnmt3c but not Miwi2 mutants. TEX15 is associated with MILI in testis. As loss of Tex15 causes TE desilencing with intact piRNA production, our results identify TEX15 as a new essential epigenetic regulator that may function as a nuclear effector of MILI to silence TEs by DNA methylation.
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spelling pubmed-72631412020-12-01 TEX15 associates with MILI and silences transposable elements in male germ cells Yang, Fang Lan, Yemin Pandey, Radha Raman Homolka, David Berger, Shelley L. Pillai, Ramesh S. Bartolomei, Marisa S. Wang, P. Jeremy Genes Dev Research Communication DNA methylation is a major silencing mechanism of transposable elements (TEs). Here we report that TEX15, a testis-specific protein, is required for TE silencing. TEX15 is expressed in embryonic germ cells and functions during genome-wide epigenetic reprogramming. The Tex15 mutant exhibits DNA hypomethylation in TEs at a level similar to Mili and Dnmt3c but not Miwi2 mutants. TEX15 is associated with MILI in testis. As loss of Tex15 causes TE desilencing with intact piRNA production, our results identify TEX15 as a new essential epigenetic regulator that may function as a nuclear effector of MILI to silence TEs by DNA methylation. Cold Spring Harbor Laboratory Press 2020-06-01 /pmc/articles/PMC7263141/ /pubmed/32381626 http://dx.doi.org/10.1101/gad.335489.119 Text en © 2020 Yang et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Communication
Yang, Fang
Lan, Yemin
Pandey, Radha Raman
Homolka, David
Berger, Shelley L.
Pillai, Ramesh S.
Bartolomei, Marisa S.
Wang, P. Jeremy
TEX15 associates with MILI and silences transposable elements in male germ cells
title TEX15 associates with MILI and silences transposable elements in male germ cells
title_full TEX15 associates with MILI and silences transposable elements in male germ cells
title_fullStr TEX15 associates with MILI and silences transposable elements in male germ cells
title_full_unstemmed TEX15 associates with MILI and silences transposable elements in male germ cells
title_short TEX15 associates with MILI and silences transposable elements in male germ cells
title_sort tex15 associates with mili and silences transposable elements in male germ cells
topic Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263141/
https://www.ncbi.nlm.nih.gov/pubmed/32381626
http://dx.doi.org/10.1101/gad.335489.119
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