Genome-wide CRISPR screening reveals genes essential for cell viability and resistance to abiotic and biotic stresses in Bombyx mori

High-throughput genetic screens are powerful methods to interrogate gene function on a genome-wide scale and identify genes responsible to certain stresses. Here, we developed a piggyBac strategy to deliver pooled sgRNA libraries stably into cell lines. We used this strategy to conduct a screen base...

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Detalles Bibliográficos
Autores principales: Chang, Jiasong, Wang, Ruolin, Yu, Kai, Zhang, Tong, Chen, Xiaoxu, Liu, Yue, Shi, Run, Wang, Xiaogang, Xia, Qingyou, Ma, Sanyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263191/
https://www.ncbi.nlm.nih.gov/pubmed/32424075
http://dx.doi.org/10.1101/gr.249045.119

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7263191/
https://www.ncbi.nlm.nih.gov/pubmed/32424075
http://dx.doi.org/10.1101/gr.249045.119

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