Droplet digital polymerase chain reaction-based quantification of circulating microRNAs using small RNA concentration normalization

Quantification of plasma microRNAs (miRNAs) as non-invasive disease biomarkers is subject to multiple technical variabilities. This study aimed to develop an optimized protocol for miRNA quantification from rodent plasma. We hypothesized that a fixed small RNA concentration input for reverse transcr...

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Detalles Bibliográficos
Autores principales: Das Gupta, Shalini, Ndode-Ekane, Xavier Ekolle, Puhakka, Noora, Pitkänen, Asla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265372/
https://www.ncbi.nlm.nih.gov/pubmed/32488181
http://dx.doi.org/10.1038/s41598-020-66072-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265372/
https://www.ncbi.nlm.nih.gov/pubmed/32488181
http://dx.doi.org/10.1038/s41598-020-66072-z

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