CRISPR‐TAPE: protein‐centric CRISPR guide design for targeted proteome engineering

Rational molecular engineering of proteins with CRISPR‐based approaches is challenged by the gene‐centric nature of gRNA design tools. To address this, we have developed CRISPR‐TAPE, a protein‐centric gRNA design algorithm that allows users to target specific residues, or amino acid types within pro...

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Detalles Bibliográficos
Autores principales: Anderson, Daniel Paolo, Benns, Henry James, Tate, Edward William, Child, Matthew Andrew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266498/
https://www.ncbi.nlm.nih.gov/pubmed/32485092
http://dx.doi.org/10.15252/msb.20209475

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266498/
https://www.ncbi.nlm.nih.gov/pubmed/32485092
http://dx.doi.org/10.15252/msb.20209475

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