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The fiber metabolite butyrate reduces gp130 by targeting TRAF5 in colorectal cancer cells
BACKGROUND: Dietary fiber is effective for colorectal cancer (CRC) treatment. Interleukin-6 (IL-6) and its adaptors are potential targets for CRC therapy. Butyrate, a metabolite of dietary fiber, is a new, highly safe type of targeted drug. METHODS: In this study, Cell Counting Kit-8 cell viability...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271451/ https://www.ncbi.nlm.nih.gov/pubmed/32518521 http://dx.doi.org/10.1186/s12935-020-01305-9 |
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author | Yuan, Yin Li, Bo Kuang, Yanbin Ni, Shuo Zhuge, Aoxiang Yang, Jing Lv, Longxian Gu, Silan Yan, Ren Li, Yating Wang, Kaicen Yang, Liya Zhu, Xueling Wu, Jingjing Bian, Xiaoyuan Li, Lanjuan |
author_facet | Yuan, Yin Li, Bo Kuang, Yanbin Ni, Shuo Zhuge, Aoxiang Yang, Jing Lv, Longxian Gu, Silan Yan, Ren Li, Yating Wang, Kaicen Yang, Liya Zhu, Xueling Wu, Jingjing Bian, Xiaoyuan Li, Lanjuan |
author_sort | Yuan, Yin |
collection | PubMed |
description | BACKGROUND: Dietary fiber is effective for colorectal cancer (CRC) treatment. Interleukin-6 (IL-6) and its adaptors are potential targets for CRC therapy. Butyrate, a metabolite of dietary fiber, is a new, highly safe type of targeted drug. METHODS: In this study, Cell Counting Kit-8 cell viability and wound healing assays, western blot analysis, immunofluorescence staining, and xenograft tumor mouse models were used to evaluate the anticancer effect of butyrate and its possible mechanism in vivo and in vitro. RESULTS: Dietary fiber and sodium butyrate (NaB) decreased CRC burden by decreasing IL-6 receptor gp130 and blocking IL-6/JAK2/STAT3 axis activation in vitro and in vivo. Furthermore, NaB reduced the gp130 protein level by regulating its degradation rate via targeting TRAF5. CONCLUSIONS: The fiber metabolite butyrate inhibits CRC development by reducing gp130 via TRAF5. |
format | Online Article Text |
id | pubmed-7271451 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-72714512020-06-08 The fiber metabolite butyrate reduces gp130 by targeting TRAF5 in colorectal cancer cells Yuan, Yin Li, Bo Kuang, Yanbin Ni, Shuo Zhuge, Aoxiang Yang, Jing Lv, Longxian Gu, Silan Yan, Ren Li, Yating Wang, Kaicen Yang, Liya Zhu, Xueling Wu, Jingjing Bian, Xiaoyuan Li, Lanjuan Cancer Cell Int Primary Research BACKGROUND: Dietary fiber is effective for colorectal cancer (CRC) treatment. Interleukin-6 (IL-6) and its adaptors are potential targets for CRC therapy. Butyrate, a metabolite of dietary fiber, is a new, highly safe type of targeted drug. METHODS: In this study, Cell Counting Kit-8 cell viability and wound healing assays, western blot analysis, immunofluorescence staining, and xenograft tumor mouse models were used to evaluate the anticancer effect of butyrate and its possible mechanism in vivo and in vitro. RESULTS: Dietary fiber and sodium butyrate (NaB) decreased CRC burden by decreasing IL-6 receptor gp130 and blocking IL-6/JAK2/STAT3 axis activation in vitro and in vivo. Furthermore, NaB reduced the gp130 protein level by regulating its degradation rate via targeting TRAF5. CONCLUSIONS: The fiber metabolite butyrate inhibits CRC development by reducing gp130 via TRAF5. BioMed Central 2020-06-03 /pmc/articles/PMC7271451/ /pubmed/32518521 http://dx.doi.org/10.1186/s12935-020-01305-9 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Primary Research Yuan, Yin Li, Bo Kuang, Yanbin Ni, Shuo Zhuge, Aoxiang Yang, Jing Lv, Longxian Gu, Silan Yan, Ren Li, Yating Wang, Kaicen Yang, Liya Zhu, Xueling Wu, Jingjing Bian, Xiaoyuan Li, Lanjuan The fiber metabolite butyrate reduces gp130 by targeting TRAF5 in colorectal cancer cells |
title | The fiber metabolite butyrate reduces gp130 by targeting TRAF5 in colorectal cancer cells |
title_full | The fiber metabolite butyrate reduces gp130 by targeting TRAF5 in colorectal cancer cells |
title_fullStr | The fiber metabolite butyrate reduces gp130 by targeting TRAF5 in colorectal cancer cells |
title_full_unstemmed | The fiber metabolite butyrate reduces gp130 by targeting TRAF5 in colorectal cancer cells |
title_short | The fiber metabolite butyrate reduces gp130 by targeting TRAF5 in colorectal cancer cells |
title_sort | fiber metabolite butyrate reduces gp130 by targeting traf5 in colorectal cancer cells |
topic | Primary Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271451/ https://www.ncbi.nlm.nih.gov/pubmed/32518521 http://dx.doi.org/10.1186/s12935-020-01305-9 |
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