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Big data from small tissues: extraction of high-quality RNA for RNA-sequencing from different oilseed Brassica seed tissues during seed development

BACKGROUND: Obtaining high-quality RNA for gene expression analyses from different seed tissues is challenging due to the presence of various contaminants, such as polyphenols, polysaccharides and lipids which interfere with RNA extraction methods. At present, the available protocols for extracting...

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Detalles Bibliográficos
Autores principales: Siles, Laura, Eastmond, Peter, Kurup, Smita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275424/
https://www.ncbi.nlm.nih.gov/pubmed/32518582
http://dx.doi.org/10.1186/s13007-020-00626-0