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Host nutrient milieu drives an essential role for aspartate biosynthesis during invasive Staphylococcus aureus infection
The bacterial pathogen Staphylococcus aureus is capable of infecting a broad spectrum of host tissues, in part due to flexibility of metabolic programs. S. aureus, like all organisms, requires essential biosynthetic intermediates to synthesize macromolecules. We therefore sought to determine the met...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275739/ https://www.ncbi.nlm.nih.gov/pubmed/32414924 http://dx.doi.org/10.1073/pnas.1922211117 |
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author | Potter, Aimee D. Butrico, Casey E. Ford, Caleb A. Curry, Jacob M. Trenary, Irina A. Tummarakota, Srivarun S. Hendrix, Andrew S. Young, Jamey D. Cassat, James E. |
author_facet | Potter, Aimee D. Butrico, Casey E. Ford, Caleb A. Curry, Jacob M. Trenary, Irina A. Tummarakota, Srivarun S. Hendrix, Andrew S. Young, Jamey D. Cassat, James E. |
author_sort | Potter, Aimee D. |
collection | PubMed |
description | The bacterial pathogen Staphylococcus aureus is capable of infecting a broad spectrum of host tissues, in part due to flexibility of metabolic programs. S. aureus, like all organisms, requires essential biosynthetic intermediates to synthesize macromolecules. We therefore sought to determine the metabolic pathways contributing to synthesis of essential precursors during invasive S. aureus infection. We focused specifically on staphylococcal infection of bone, one of the most common sites of invasive S. aureus infection and a unique environment characterized by dynamic substrate accessibility, infection-induced hypoxia, and a metabolic profile skewed toward aerobic glycolysis. Using a murine model of osteomyelitis, we examined survival of S. aureus mutants deficient in central metabolic pathways, including glycolysis, gluconeogenesis, the tricarboxylic acid (TCA) cycle, and amino acid synthesis/catabolism. Despite the high glycolytic demand of skeletal cells, we discovered that S. aureus requires glycolysis for survival in bone. Furthermore, the TCA cycle is dispensable for survival during osteomyelitis, and S. aureus instead has a critical need for anaplerosis. Bacterial synthesis of aspartate in particular is absolutely essential for staphylococcal survival in bone, despite the presence of an aspartate transporter, which we identified as GltT and confirmed biochemically. This dependence on endogenous aspartate synthesis derives from the presence of excess glutamate in infected tissue, which inhibits aspartate acquisition by S. aureus. Together, these data elucidate the metabolic pathways required for staphylococcal infection within bone and demonstrate that the host nutrient milieu can determine essentiality of bacterial nutrient biosynthesis pathways despite the presence of dedicated transporters. |
format | Online Article Text |
id | pubmed-7275739 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-72757392020-06-11 Host nutrient milieu drives an essential role for aspartate biosynthesis during invasive Staphylococcus aureus infection Potter, Aimee D. Butrico, Casey E. Ford, Caleb A. Curry, Jacob M. Trenary, Irina A. Tummarakota, Srivarun S. Hendrix, Andrew S. Young, Jamey D. Cassat, James E. Proc Natl Acad Sci U S A Biological Sciences The bacterial pathogen Staphylococcus aureus is capable of infecting a broad spectrum of host tissues, in part due to flexibility of metabolic programs. S. aureus, like all organisms, requires essential biosynthetic intermediates to synthesize macromolecules. We therefore sought to determine the metabolic pathways contributing to synthesis of essential precursors during invasive S. aureus infection. We focused specifically on staphylococcal infection of bone, one of the most common sites of invasive S. aureus infection and a unique environment characterized by dynamic substrate accessibility, infection-induced hypoxia, and a metabolic profile skewed toward aerobic glycolysis. Using a murine model of osteomyelitis, we examined survival of S. aureus mutants deficient in central metabolic pathways, including glycolysis, gluconeogenesis, the tricarboxylic acid (TCA) cycle, and amino acid synthesis/catabolism. Despite the high glycolytic demand of skeletal cells, we discovered that S. aureus requires glycolysis for survival in bone. Furthermore, the TCA cycle is dispensable for survival during osteomyelitis, and S. aureus instead has a critical need for anaplerosis. Bacterial synthesis of aspartate in particular is absolutely essential for staphylococcal survival in bone, despite the presence of an aspartate transporter, which we identified as GltT and confirmed biochemically. This dependence on endogenous aspartate synthesis derives from the presence of excess glutamate in infected tissue, which inhibits aspartate acquisition by S. aureus. Together, these data elucidate the metabolic pathways required for staphylococcal infection within bone and demonstrate that the host nutrient milieu can determine essentiality of bacterial nutrient biosynthesis pathways despite the presence of dedicated transporters. National Academy of Sciences 2020-06-02 2020-05-15 /pmc/articles/PMC7275739/ /pubmed/32414924 http://dx.doi.org/10.1073/pnas.1922211117 Text en Copyright © 2020 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Potter, Aimee D. Butrico, Casey E. Ford, Caleb A. Curry, Jacob M. Trenary, Irina A. Tummarakota, Srivarun S. Hendrix, Andrew S. Young, Jamey D. Cassat, James E. Host nutrient milieu drives an essential role for aspartate biosynthesis during invasive Staphylococcus aureus infection |
title | Host nutrient milieu drives an essential role for aspartate biosynthesis during invasive Staphylococcus aureus infection |
title_full | Host nutrient milieu drives an essential role for aspartate biosynthesis during invasive Staphylococcus aureus infection |
title_fullStr | Host nutrient milieu drives an essential role for aspartate biosynthesis during invasive Staphylococcus aureus infection |
title_full_unstemmed | Host nutrient milieu drives an essential role for aspartate biosynthesis during invasive Staphylococcus aureus infection |
title_short | Host nutrient milieu drives an essential role for aspartate biosynthesis during invasive Staphylococcus aureus infection |
title_sort | host nutrient milieu drives an essential role for aspartate biosynthesis during invasive staphylococcus aureus infection |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7275739/ https://www.ncbi.nlm.nih.gov/pubmed/32414924 http://dx.doi.org/10.1073/pnas.1922211117 |
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