Cargando…

UNAGI: an automated pipeline for nanopore full-length cDNA sequencing uncovers novel transcripts and isoforms in yeast

Sequencing the entire RNA molecule leads to a better understanding of the transcriptome architecture. SMARTer (Switching Mechanism at 5′-End of RNA Template) is a technology aimed at generating full-length cDNA from low amounts of mRNA for sequencing by short-read sequencers such as those from Illum...

Descripción completa

Detalles Bibliográficos
Autores principales: Al kadi, Mohamad, Jung, Nicolas, Ito, Shingo, Kameoka, Shoichiro, Hishida, Takashi, Motooka, Daisuke, Nakamura, Shota, Iida, Tetsuya, Okuzaki, Daisuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283198/
https://www.ncbi.nlm.nih.gov/pubmed/31955296
http://dx.doi.org/10.1007/s10142-020-00732-1