Anoxic cell rupture of Prevotella bryantii by high-pressure homogenization protects the Na(+)-translocating NADH:quinone oxidoreductase from oxidative damage

Respiratory NADH oxidation in the rumen bacterium Prevotella bryantii is catalyzed by the Na(+)-translocating NADH:quinone oxidoreductase (NQR). A method for cell disruption and membrane isolation of P. bryantii under anoxic conditions using the EmulisFlex-C3 homogenizer is described. We compared NQ...

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Detalles Bibliográficos
Autores principales: Schleicher, Lena, Fritz, Günter, Seifert, Jana, Steuber, Julia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283201/
https://www.ncbi.nlm.nih.gov/pubmed/31955240
http://dx.doi.org/10.1007/s00203-019-01805-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283201/
https://www.ncbi.nlm.nih.gov/pubmed/31955240
http://dx.doi.org/10.1007/s00203-019-01805-x

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