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What's the norm in normalization? A frightening note on the use of RT-qPCR in the livestock science
Reverse-Transcription quantitative PCR (RT-qPCR) provides a valuable tool to study gene expression with exquisite sensitivity. To retain its inferential power, user-introduced technical variability must be reduced and accounted for. Selecting a set of stably expressed internal control genes (ICG), v...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285961/ https://www.ncbi.nlm.nih.gov/pubmed/34531001 http://dx.doi.org/10.1016/j.gene.2018.100003 |