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What's the norm in normalization? A frightening note on the use of RT-qPCR in the livestock science

Reverse-Transcription quantitative PCR (RT-qPCR) provides a valuable tool to study gene expression with exquisite sensitivity. To retain its inferential power, user-introduced technical variability must be reduced and accounted for. Selecting a set of stably expressed internal control genes (ICG), v...

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Detalles Bibliográficos
Autores principales: Busato, Sebastiano, Mezzetti, Matteo, Logan, Paul, Aguilera, Nicolas, Bionaz, Massimo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285961/
https://www.ncbi.nlm.nih.gov/pubmed/34531001
http://dx.doi.org/10.1016/j.gene.2018.100003