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Aquaporin 1 elicits cell motility and coordinates vascular bed formation by downregulating thrombospondin type‐1 domain‐containing 7A in glioblastoma

BACKGROUND: Aquaporin (AQP) 1 expression has been linked with tumor malignancy but its role in glioblastoma (GBM), a lethal glioma, remains to be clarified. METHODS: AQP1 expression was examined in 33 human GBM specimens by immunohistochemistry. GBM cells (U251 and U87) that stably express AQP1 were...

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Autores principales: Oishi, Masahiro, Munesue, Seiichi, Harashima, Ai, Nakada, Mitsutoshi, Yamamoto, Yasuhiko, Hayashi, Yasuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286445/
https://www.ncbi.nlm.nih.gov/pubmed/32253832
http://dx.doi.org/10.1002/cam4.3032
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author Oishi, Masahiro
Munesue, Seiichi
Harashima, Ai
Nakada, Mitsutoshi
Yamamoto, Yasuhiko
Hayashi, Yasuhiko
author_facet Oishi, Masahiro
Munesue, Seiichi
Harashima, Ai
Nakada, Mitsutoshi
Yamamoto, Yasuhiko
Hayashi, Yasuhiko
author_sort Oishi, Masahiro
collection PubMed
description BACKGROUND: Aquaporin (AQP) 1 expression has been linked with tumor malignancy but its role in glioblastoma (GBM), a lethal glioma, remains to be clarified. METHODS: AQP1 expression was examined in 33 human GBM specimens by immunohistochemistry. GBM cells (U251 and U87) that stably express AQP1 were established and used for cellular proliferation, migration, invasion, and vascular tube formation assays. The GeneChip assay was used to identify differentially expressed genes in AQP1‐expressing cells. RESULTS: AQP1 was expressed only in tumor cells. AQP1 dose‐dependently accelerated cell migration and invasion, but not proliferation, in GBM cell lines. AQP1 also upregulated cathepsin B, focal adhesion kinase and activities of matrix metalloproteinase 9. AQP1 in GBM cells induced wall thickness of ECV304, vascular endothelial cells, in a contact‐dependent manner. Downregulation of thrombospondin type 1 domain containing 7A (THSD7A) was identified in AQP1‐expressing GBM cells in vitro, and was negatively correlated with AQP1 expression in human GBM specimens. CONCLUSION: AQP1 is involved in tumor malignancy by facilitating the migration and invasion of GBM cells, and promoting the formation of vascular beds that are characteristic of GBM by downregulating THSD7A.
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spelling pubmed-72864452020-06-11 Aquaporin 1 elicits cell motility and coordinates vascular bed formation by downregulating thrombospondin type‐1 domain‐containing 7A in glioblastoma Oishi, Masahiro Munesue, Seiichi Harashima, Ai Nakada, Mitsutoshi Yamamoto, Yasuhiko Hayashi, Yasuhiko Cancer Med Cancer Biology BACKGROUND: Aquaporin (AQP) 1 expression has been linked with tumor malignancy but its role in glioblastoma (GBM), a lethal glioma, remains to be clarified. METHODS: AQP1 expression was examined in 33 human GBM specimens by immunohistochemistry. GBM cells (U251 and U87) that stably express AQP1 were established and used for cellular proliferation, migration, invasion, and vascular tube formation assays. The GeneChip assay was used to identify differentially expressed genes in AQP1‐expressing cells. RESULTS: AQP1 was expressed only in tumor cells. AQP1 dose‐dependently accelerated cell migration and invasion, but not proliferation, in GBM cell lines. AQP1 also upregulated cathepsin B, focal adhesion kinase and activities of matrix metalloproteinase 9. AQP1 in GBM cells induced wall thickness of ECV304, vascular endothelial cells, in a contact‐dependent manner. Downregulation of thrombospondin type 1 domain containing 7A (THSD7A) was identified in AQP1‐expressing GBM cells in vitro, and was negatively correlated with AQP1 expression in human GBM specimens. CONCLUSION: AQP1 is involved in tumor malignancy by facilitating the migration and invasion of GBM cells, and promoting the formation of vascular beds that are characteristic of GBM by downregulating THSD7A. John Wiley and Sons Inc. 2020-04-06 /pmc/articles/PMC7286445/ /pubmed/32253832 http://dx.doi.org/10.1002/cam4.3032 Text en © 2020 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Cancer Biology
Oishi, Masahiro
Munesue, Seiichi
Harashima, Ai
Nakada, Mitsutoshi
Yamamoto, Yasuhiko
Hayashi, Yasuhiko
Aquaporin 1 elicits cell motility and coordinates vascular bed formation by downregulating thrombospondin type‐1 domain‐containing 7A in glioblastoma
title Aquaporin 1 elicits cell motility and coordinates vascular bed formation by downregulating thrombospondin type‐1 domain‐containing 7A in glioblastoma
title_full Aquaporin 1 elicits cell motility and coordinates vascular bed formation by downregulating thrombospondin type‐1 domain‐containing 7A in glioblastoma
title_fullStr Aquaporin 1 elicits cell motility and coordinates vascular bed formation by downregulating thrombospondin type‐1 domain‐containing 7A in glioblastoma
title_full_unstemmed Aquaporin 1 elicits cell motility and coordinates vascular bed formation by downregulating thrombospondin type‐1 domain‐containing 7A in glioblastoma
title_short Aquaporin 1 elicits cell motility and coordinates vascular bed formation by downregulating thrombospondin type‐1 domain‐containing 7A in glioblastoma
title_sort aquaporin 1 elicits cell motility and coordinates vascular bed formation by downregulating thrombospondin type‐1 domain‐containing 7a in glioblastoma
topic Cancer Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286445/
https://www.ncbi.nlm.nih.gov/pubmed/32253832
http://dx.doi.org/10.1002/cam4.3032
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