Cargando…

Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix

Adhesion of T. cruzi trypomastigotes to components of the extracellular matrix (ECM) is an important step in mammalian host cell invasion. We have recently described a significant increase in the tyrosine nitration levels of histones H2A and H4 when trypomastigotes are incubated with components of t...

Descripción completa

Detalles Bibliográficos
Autores principales: Magalhães, Rubens Daniel Miserani, Mattos, Eliciane Cevolani, Rozanski, Andrei, Galante, Pedro Alexandre Favoretto, Palmisano, Giuseppe, Cruz, Angela Kaysel, Colli, Walter, Camargo, Anamaria Aranha, Alves, Maria Júlia Manso
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286532/
https://www.ncbi.nlm.nih.gov/pubmed/32469928
http://dx.doi.org/10.1371/journal.pntd.0008262
_version_ 1783544897851097088
author Magalhães, Rubens Daniel Miserani
Mattos, Eliciane Cevolani
Rozanski, Andrei
Galante, Pedro Alexandre Favoretto
Palmisano, Giuseppe
Cruz, Angela Kaysel
Colli, Walter
Camargo, Anamaria Aranha
Alves, Maria Júlia Manso
author_facet Magalhães, Rubens Daniel Miserani
Mattos, Eliciane Cevolani
Rozanski, Andrei
Galante, Pedro Alexandre Favoretto
Palmisano, Giuseppe
Cruz, Angela Kaysel
Colli, Walter
Camargo, Anamaria Aranha
Alves, Maria Júlia Manso
author_sort Magalhães, Rubens Daniel Miserani
collection PubMed
description Adhesion of T. cruzi trypomastigotes to components of the extracellular matrix (ECM) is an important step in mammalian host cell invasion. We have recently described a significant increase in the tyrosine nitration levels of histones H2A and H4 when trypomastigotes are incubated with components of the ECM. In this work, we used chromatin immunoprecipitation (ChIP) with an anti-nitrotyrosine antibody followed by mass spectrometry to identify nitrated DNA binding proteins in T. cruzi and to detect alterations in nitration levels induced upon parasite incubation with the ECM. Histone H1, H2B, H2A and H3 were detected among the 9 most abundant nitrated DNA binding proteins using this proteomic approach. One nitrated tyrosine residue (Y29) was identified in Histone H2B in the MS/MS spectrum. In addition, we observed a significant increase in the nitration levels of histones H1, H2B, H2A and H4 upon parasite incubation with ECM. Finally, we used ChIP-Seq to map global changes in the DNA binding profile of nitrated proteins. We observed a significant change in the binding pattern of nitrated proteins to DNA after parasite incubation with ECM. This work provides the first global profile of nitrated DNA binding proteins in T. cruzi and additional evidence for modification in the nitration profile of histones upon parasite incubation with ECM. Our data also indicate that the parasite interaction with the ECM induces alterations in chromatin structure, possibly affecting nuclear functions.
format Online
Article
Text
id pubmed-7286532
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-72865322020-06-15 Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix Magalhães, Rubens Daniel Miserani Mattos, Eliciane Cevolani Rozanski, Andrei Galante, Pedro Alexandre Favoretto Palmisano, Giuseppe Cruz, Angela Kaysel Colli, Walter Camargo, Anamaria Aranha Alves, Maria Júlia Manso PLoS Negl Trop Dis Research Article Adhesion of T. cruzi trypomastigotes to components of the extracellular matrix (ECM) is an important step in mammalian host cell invasion. We have recently described a significant increase in the tyrosine nitration levels of histones H2A and H4 when trypomastigotes are incubated with components of the ECM. In this work, we used chromatin immunoprecipitation (ChIP) with an anti-nitrotyrosine antibody followed by mass spectrometry to identify nitrated DNA binding proteins in T. cruzi and to detect alterations in nitration levels induced upon parasite incubation with the ECM. Histone H1, H2B, H2A and H3 were detected among the 9 most abundant nitrated DNA binding proteins using this proteomic approach. One nitrated tyrosine residue (Y29) was identified in Histone H2B in the MS/MS spectrum. In addition, we observed a significant increase in the nitration levels of histones H1, H2B, H2A and H4 upon parasite incubation with ECM. Finally, we used ChIP-Seq to map global changes in the DNA binding profile of nitrated proteins. We observed a significant change in the binding pattern of nitrated proteins to DNA after parasite incubation with ECM. This work provides the first global profile of nitrated DNA binding proteins in T. cruzi and additional evidence for modification in the nitration profile of histones upon parasite incubation with ECM. Our data also indicate that the parasite interaction with the ECM induces alterations in chromatin structure, possibly affecting nuclear functions. Public Library of Science 2020-05-29 /pmc/articles/PMC7286532/ /pubmed/32469928 http://dx.doi.org/10.1371/journal.pntd.0008262 Text en © 2020 Magalhães et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Magalhães, Rubens Daniel Miserani
Mattos, Eliciane Cevolani
Rozanski, Andrei
Galante, Pedro Alexandre Favoretto
Palmisano, Giuseppe
Cruz, Angela Kaysel
Colli, Walter
Camargo, Anamaria Aranha
Alves, Maria Júlia Manso
Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix
title Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix
title_full Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix
title_fullStr Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix
title_full_unstemmed Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix
title_short Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix
title_sort global changes in nitration levels and dna binding profile of trypanosoma cruzi histones induced by incubation with host extracellular matrix
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286532/
https://www.ncbi.nlm.nih.gov/pubmed/32469928
http://dx.doi.org/10.1371/journal.pntd.0008262
work_keys_str_mv AT magalhaesrubensdanielmiserani globalchangesinnitrationlevelsanddnabindingprofileoftrypanosomacruzihistonesinducedbyincubationwithhostextracellularmatrix
AT mattoselicianecevolani globalchangesinnitrationlevelsanddnabindingprofileoftrypanosomacruzihistonesinducedbyincubationwithhostextracellularmatrix
AT rozanskiandrei globalchangesinnitrationlevelsanddnabindingprofileoftrypanosomacruzihistonesinducedbyincubationwithhostextracellularmatrix
AT galantepedroalexandrefavoretto globalchangesinnitrationlevelsanddnabindingprofileoftrypanosomacruzihistonesinducedbyincubationwithhostextracellularmatrix
AT palmisanogiuseppe globalchangesinnitrationlevelsanddnabindingprofileoftrypanosomacruzihistonesinducedbyincubationwithhostextracellularmatrix
AT cruzangelakaysel globalchangesinnitrationlevelsanddnabindingprofileoftrypanosomacruzihistonesinducedbyincubationwithhostextracellularmatrix
AT colliwalter globalchangesinnitrationlevelsanddnabindingprofileoftrypanosomacruzihistonesinducedbyincubationwithhostextracellularmatrix
AT camargoanamariaaranha globalchangesinnitrationlevelsanddnabindingprofileoftrypanosomacruzihistonesinducedbyincubationwithhostextracellularmatrix
AT alvesmariajuliamanso globalchangesinnitrationlevelsanddnabindingprofileoftrypanosomacruzihistonesinducedbyincubationwithhostextracellularmatrix