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Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix
Adhesion of T. cruzi trypomastigotes to components of the extracellular matrix (ECM) is an important step in mammalian host cell invasion. We have recently described a significant increase in the tyrosine nitration levels of histones H2A and H4 when trypomastigotes are incubated with components of t...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286532/ https://www.ncbi.nlm.nih.gov/pubmed/32469928 http://dx.doi.org/10.1371/journal.pntd.0008262 |
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author | Magalhães, Rubens Daniel Miserani Mattos, Eliciane Cevolani Rozanski, Andrei Galante, Pedro Alexandre Favoretto Palmisano, Giuseppe Cruz, Angela Kaysel Colli, Walter Camargo, Anamaria Aranha Alves, Maria Júlia Manso |
author_facet | Magalhães, Rubens Daniel Miserani Mattos, Eliciane Cevolani Rozanski, Andrei Galante, Pedro Alexandre Favoretto Palmisano, Giuseppe Cruz, Angela Kaysel Colli, Walter Camargo, Anamaria Aranha Alves, Maria Júlia Manso |
author_sort | Magalhães, Rubens Daniel Miserani |
collection | PubMed |
description | Adhesion of T. cruzi trypomastigotes to components of the extracellular matrix (ECM) is an important step in mammalian host cell invasion. We have recently described a significant increase in the tyrosine nitration levels of histones H2A and H4 when trypomastigotes are incubated with components of the ECM. In this work, we used chromatin immunoprecipitation (ChIP) with an anti-nitrotyrosine antibody followed by mass spectrometry to identify nitrated DNA binding proteins in T. cruzi and to detect alterations in nitration levels induced upon parasite incubation with the ECM. Histone H1, H2B, H2A and H3 were detected among the 9 most abundant nitrated DNA binding proteins using this proteomic approach. One nitrated tyrosine residue (Y29) was identified in Histone H2B in the MS/MS spectrum. In addition, we observed a significant increase in the nitration levels of histones H1, H2B, H2A and H4 upon parasite incubation with ECM. Finally, we used ChIP-Seq to map global changes in the DNA binding profile of nitrated proteins. We observed a significant change in the binding pattern of nitrated proteins to DNA after parasite incubation with ECM. This work provides the first global profile of nitrated DNA binding proteins in T. cruzi and additional evidence for modification in the nitration profile of histones upon parasite incubation with ECM. Our data also indicate that the parasite interaction with the ECM induces alterations in chromatin structure, possibly affecting nuclear functions. |
format | Online Article Text |
id | pubmed-7286532 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-72865322020-06-15 Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix Magalhães, Rubens Daniel Miserani Mattos, Eliciane Cevolani Rozanski, Andrei Galante, Pedro Alexandre Favoretto Palmisano, Giuseppe Cruz, Angela Kaysel Colli, Walter Camargo, Anamaria Aranha Alves, Maria Júlia Manso PLoS Negl Trop Dis Research Article Adhesion of T. cruzi trypomastigotes to components of the extracellular matrix (ECM) is an important step in mammalian host cell invasion. We have recently described a significant increase in the tyrosine nitration levels of histones H2A and H4 when trypomastigotes are incubated with components of the ECM. In this work, we used chromatin immunoprecipitation (ChIP) with an anti-nitrotyrosine antibody followed by mass spectrometry to identify nitrated DNA binding proteins in T. cruzi and to detect alterations in nitration levels induced upon parasite incubation with the ECM. Histone H1, H2B, H2A and H3 were detected among the 9 most abundant nitrated DNA binding proteins using this proteomic approach. One nitrated tyrosine residue (Y29) was identified in Histone H2B in the MS/MS spectrum. In addition, we observed a significant increase in the nitration levels of histones H1, H2B, H2A and H4 upon parasite incubation with ECM. Finally, we used ChIP-Seq to map global changes in the DNA binding profile of nitrated proteins. We observed a significant change in the binding pattern of nitrated proteins to DNA after parasite incubation with ECM. This work provides the first global profile of nitrated DNA binding proteins in T. cruzi and additional evidence for modification in the nitration profile of histones upon parasite incubation with ECM. Our data also indicate that the parasite interaction with the ECM induces alterations in chromatin structure, possibly affecting nuclear functions. Public Library of Science 2020-05-29 /pmc/articles/PMC7286532/ /pubmed/32469928 http://dx.doi.org/10.1371/journal.pntd.0008262 Text en © 2020 Magalhães et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Magalhães, Rubens Daniel Miserani Mattos, Eliciane Cevolani Rozanski, Andrei Galante, Pedro Alexandre Favoretto Palmisano, Giuseppe Cruz, Angela Kaysel Colli, Walter Camargo, Anamaria Aranha Alves, Maria Júlia Manso Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix |
title | Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix |
title_full | Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix |
title_fullStr | Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix |
title_full_unstemmed | Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix |
title_short | Global changes in nitration levels and DNA binding profile of Trypanosoma cruzi histones induced by incubation with host extracellular matrix |
title_sort | global changes in nitration levels and dna binding profile of trypanosoma cruzi histones induced by incubation with host extracellular matrix |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7286532/ https://www.ncbi.nlm.nih.gov/pubmed/32469928 http://dx.doi.org/10.1371/journal.pntd.0008262 |
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