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Establishment and transcriptomic analyses of a cattle rumen epithelial primary cells (REPC) culture by bulk and single-cell RNA sequencing to elucidate interactions of butyrate and rumen development

As a critical and high-value tool to study the development of rumen, we established a stable rumen epithelial primary cell (REPC) culture from a two-week-old Holstein bull calf rumen epithelial tissue. The transcriptomic profiling of the REPC and the direct effects of butyrate on gene expression wer...

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Detalles Bibliográficos
Autores principales: Lin, Shudai, Fang, Lingzhao, Kang, Xiaolong, Liu, Shuli, Liu, Mei, Connor, Erin E., Baldwin, Ransom L., Liu, George, Li, Cong-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287249/
https://www.ncbi.nlm.nih.gov/pubmed/32551379
http://dx.doi.org/10.1016/j.heliyon.2020.e04112