Efficient Generation and Correction of Mutations in Human iPS Cells Utilizing mRNAs of CRISPR Base Editors and Prime Editors

In contrast to CRISPR/Cas9 nucleases, CRISPR base editors (BE) and prime editors (PE) enable predefined nucleotide exchanges in genomic sequences without generating DNA double strand breaks. Here, we employed BE and PE mRNAs in conjunction with chemically synthesized sgRNAs and pegRNAs for efficient...

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Detalles Bibliográficos
Autores principales: Sürün, Duran, Schneider, Aksana, Mircetic, Jovan, Neumann, Katrin, Lansing, Felix, Paszkowski-Rogacz, Maciej, Hänchen, Vanessa, Lee-Kirsch, Min Ae, Buchholz, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7288465/
https://www.ncbi.nlm.nih.gov/pubmed/32384610
http://dx.doi.org/10.3390/genes11050511

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7288465/
https://www.ncbi.nlm.nih.gov/pubmed/32384610
http://dx.doi.org/10.3390/genes11050511

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