Calcium-Free and Cytochalasin B Treatment Inhibits Blastomere Fusion in 2-Cell Stage Embryos for the Generation of Floxed Mice via Sequential Electroporation

The generation of conditional knockout mice using the Cre-loxP system is advantageous for the functional analysis of genes. Flanked by two loxP sites (floxed) mice can be directly obtained from fertilized eggs by the CRISPR/Cas9 genome editing system. We previously reported that sequential knock-in...

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Detalles Bibliográficos
Autores principales: Horii, Takuro, Kobayashi, Ryosuke, Kimura, Mika, Morita, Sumiyo, Hatada, Izuho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290713/
https://www.ncbi.nlm.nih.gov/pubmed/32354036
http://dx.doi.org/10.3390/cells9051088

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290713/
https://www.ncbi.nlm.nih.gov/pubmed/32354036
http://dx.doi.org/10.3390/cells9051088

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